普伐他汀和阿司匹林对CD40-CD40L表达及斑块稳定性的影响

目的：研究普伐他汀和阿司匹林对兔腹主动脉粥样硬化斑块CD40-CD40L表达及斑块稳定性的影响。方法：新西兰大白兔32只予高脂饲养16wk及腹主动脉球囊损伤术建立腹主动脉粥样硬化模型,分4组：P组（普伐他汀）、A组（阿司匹林）、A＋P组（联合用药）与N组（不用药）,分别予相应药物灌胃8wk。通过免疫组化检测比较各组兔腹主动脉粥样硬化斑块内CD40-CD40L表达、MMP-1、胶原及脂质含量变化,超声检测腹主动脉内膜-中膜厚度（IMT）改变,ELISA法测定血清C反应蛋白（CRP）变化,分析2药干预动脉粥样硬化斑块稳定性的机制。结果：用药各组兔腹主动脉斑块内CD40-CD40L表达较N组显著受抑,普伐他汀作用更显著（P组和A＋P组均P＜0．01）；同时各用药组MMP-1的表达、IMT增厚及CRP增高较N组均得到明显抑制（P＜0．01）,P组和A＋P组较N组斑块内脂质显著减少（P＜0．01）,胶原含量明显增加（P＜0．01）。结论：普伐他汀和阿司匹林都可抑制斑块CD40-CD40L系统的表达,促进斑块的稳定性。普伐他汀促进斑块稳定的机制可能是多方面的。

Effects of pravastatin and aspirin on expression of CD40-CD40L and plaque stability

AIM:To study the effects of pravastatin and aspirin on the expression of CD40-CD40L and plaque stability.METHODS:A total of 32 New Zealand white rabbits were randomized into 4 groups after underwent balloon-induced arterial wall injury,then were given a diet of high lipid for 16 weeks followed by a high lipid diet for 1 week to establish atheroselerotie plaque model.Eight weeks after operation,rabbits were exposed to one of four treatments:no drug (N group),pravastatin (P group),aspirin (A group),combination of pravastatin and aspirin (A+P group).The expression of CD40-CD40L,MMP-1,content of collagen and lipid on plaque were measured by immunohistochemical staining,IMT was detected by ultrasound and the changes of serum CRP was tested with ELISA.RESULTS:The mechanism of drug intervention on plaque stability was analyzed.Compared with N group,the expression of CD40-CD40L on abdominal aorta plaque was significantly suppressed in other three groups,especially in P and A+P groups (P<0.01).MMP-1 expression,IMT and CRP were decreased significantly (P<0.01) in drug using groups.Lipid decreased and collagen increased significantly on plaque in P and A+P groups (P<0.01).CONCLUSION:Both pravastatin and aspirin can inhibit the expression of CD40-CD40L on plaque and promote plaque stability.The mechanism of pravastatin promoting plaque stability may be multifactorial.