7型腺病毒温州株基因组主要序列的克隆与分析

目的：克隆和分析7型腺病毒温州株（Ad7wz1）基因组主要序列ITR、E1A261R、E1B55kDa、Hexon和E3。方法：分离并提取了Ad7wz1基因组DNA,PCR扩增得到各基因后利用PMD18 simple T载体进行克隆,并测序分析。结果：获得Ad7wz1 ITR-L、E1A261R、E1B55kDa、Hexon、E3和ITR-R六个基因的克隆,并测得核苷酸序列;分析表明其核苷酸序列和相应的氨基酸序列与GenBank上已发表的Ad7全基因组序列比较同源性分别达到93.2%～100%和97.8%～100%。结论：初步确定本次分离的Ad7wz1毒株为Ad7d2型,Ad7wz1与已经发表的Ad7病毒株序列有高度同源性,为研究Ad7的致病机制提供了重要的理论基础。

Cloning and analysis of main sequences of adenovirus type 7 Wenzhou strain genome

Objective：To clone and analyze of main sequences of adenovirus type 7 Wenzhou strain genome（Ad7wz1）,including ITR,E1A261R,E1B55kDa,Hexon,and E3.Methods：Ad7wz1 ge-nome was isolated and purified,each gene putting into PMD18 simple T vector after amplified by PCR was cloned,then sequenced.Results：Six genes of Ad7wz1,including ITR-L,E1A261R,E1B55kDa,Hexon,E3 and ITR-R were cloned.The result of sequencing showed that the homology of the nucleotide sequence was 93.2%～100% and the homology of the commensurate amino acid sequence was 97.8%～100% after comparied with genomewide sequence of the Ad7 strain reported in GenBank.Conclusion：Ad7wz1 is ascertained as type Ad7d2 initialy,which shows a high homology with corresponding region of Ad7 strain reported,it affords the important theoretical basis for research on Ad7 pathogenesis.