反相高效液相法测定人血清中的罗哌卡因

 建立了一种简单、快速测定罗哌卡因血药浓度的反相高效液相法。在血清样品中加入布比卡因作内标 ,用二氯甲烷提取 ,氮气吹干 ,残渣用流动相溶解进样。条件 :分析柱为C18反相柱 ,流动相为 0 0 1mol·L-1磷酸二氢钾 (pH 3 0 ) 乙腈 (体积比为 84∶16 )溶液 ,流速为 1 2mL·min-1,在紫外检测波长 2 10nm处进行检测。罗哌卡因及内标在 11min内完全分离 ,最低检测质量浓度为 0 0 2 5mg·L-1,在 0 0 5mg·L-1～ 2 5 0mg·L-1时线性关系良好 ,r =0 9997,低、中、高浓度下的回收率、日间及日内精密度均符合方法学要求。方法简便、快速、稳定。

Determination of Ropivacaine in Human Serum by Reversed-Phase High Performance Liquid Chromatography

A simple and rapid reversed-phase high performance liquid chromatographic method was developed for the determination of ropivacaine. The sample was treated by the addition of bupivacaine as an internal standard, extraction with dichloromethane, and drying by N2. Then the residue dissolved in the mobile phase and 40 microL were injected into the chromatographic system with a mobile phase of 0.01 mol.L-1 KH2PO4 (pH 3.0)-CH3CN(84:16, volume ratio). The detection wavelength was 210 nm. The retention times of ropivacaine and the internal standard were 5.5 min and 10.5 min respectively. The detection limit was 0.025 mg.L-1. The linear range was from 0.05 mg.L-1 to 2.50 mg.L-1 with r of 0.9997. The average recovery was 104.1% with the intra-day and inter-day RSDs of 3.42% and 6.27% respectively. The results showed that it is a simple, rapid and stable method for drug monitoring in clinical research.