青春期糖尿病鼠睾丸支持细胞功能研究

４０日龄雄性Ｗｉｓｔａｒ大鼠４０只，分为对照（Ｃ）组：腹腔注射赋形剂；糖尿病（Ｄ）组：腹腔注射ＳＴＺ，５５ｍｇ／ｋｇ体重；糖尿病胰岛素及时治疗组（Ｃｏｎｔ．Ｄ）：给ＳＴＺ３天后胰岛素治疗；糖尿病胰岛素延迟治疗（Ｄｅｌ．Ｄ）组：注射ＳＴＺ３０天后胰岛素治疗，睾丸ＡＢＰ和培养支持细胞功能被研究，结果是：Ｃ，Ｃｏｎｔ．Ｄ，Ｄｅｌ．Ｄ和Ｄ组的睾丸ＡＢＰ平衡解离常数（Ｋｄ）分别是５．９１±１．７４，２．２１±０．４５，２．１３±０．７９和５．５４±１．１０ｎｍｏｌ／Ｌ，Ｃｏｎｔ．Ｄ和Ｄｅｌ．Ｄ组显著低于Ｃ和Ｄ组（Ｐ＜０．０５，ｎ＝３）；ＡＢＰ的最大结合容量（Ｂｍａｘ）分别是３１．７８±５．４７，２９．８８±３．７４，２６．７４±３．６９和２８．６９±４．５４ｆｍｏｌ／ｍｇ蛋白，组间比较差异不显著（Ｐ＞０．０５，ｎ＝３）；培养支持细胞的ＡＢＰ２４ｈ分泌量各组分别是２３．５４±５．５７，２３．６１±８．６０，１８．０６±７．５３和１４．９２±７．８９ｆｍｏｌ／１０￣６细胞，Ｄ组显著低于Ｃ和Ｃｏｎｔ．Ｄ组（Ｐ＜０．０５）。

Study of function of testicular Serroli cell in pubertal rats with diabetes

Forty male Wistar rats of forty days old were divided into 4 groups:(1)control(C):10 rats were injuected with vehicle i.p.;(2)diabetes(D):14 rates were given STZ(55 mg/kg,i.p.in 0.1mmol/l citrate buffer,pH 4.5);(3)continuous insulin replacement diabetes(Cont.D):8 dia-betic rats were given insulin replacement of 8 IU/kg insulin 3 days after STZ;(4)delayed insulin re-placement diabetes(Del.D):8 diahetic rats were given insulin replacement 30 days after STZ.Thefunction of Sertoli cell of testis was studied。The resuIts showed that:(1)the Kd values of ABP in C,Cont.D,Del.D and D were 5.91±1.74,2.21±0.45,2.13±0.79 and 5.54±1.10 nmol/Lrespectively, the values in Cont.Dand Del.Dwere significantly lower than that in C and D(P<0.05);the Bmax were 31.78±5.47,29.88±3.74,26.74±3.69 and 28.69±4.54 fmol/mg proteinrespectively.(2)the ABP production of Sertoli cell in vitro in C,Cont.D,Del.D and D were 23.54±5.57,23.61±8.60,18.06±7.53 and 14.92±7.89 fmol/106 cells/24 hours respectively,andthe D was significantly low compared with C and Cont.D(P<0.05).