合欢花水提物对大鼠脑胶质瘤C6细胞株的增殖抑制与凋亡诱导作用

采用MTT法检测合欢花水提物对C6细胞增殖活力的影响,流式细胞术检测该水提物对C6细胞的诱导凋亡作用.结果表明:合欢花水提物能抑制C6细胞的增殖,且具有剂量和时间依赖性,其在24 h、48 h、72 h时对C6细胞的半数抑制率(LD50)分别为:305 μg/mL、50 μg/mL、15 μg/mL.流式细胞术结果显示:200 tμg/mL、300μg/mL合欢花水提物均可诱导C6细胞发生早期凋亡,与对照组细胞相比,早期凋亡率分别由对照组的0.7％增加到了4.3％、14.3％.提示合欢花水提物抑制C6细胞增殖机理与诱导细胞凋亡途径有关.

Growth Inhibiting and Apoptosis-inducing Effects of the Aqueous Extract of Albizia julibrissin Durazz Flower on Rat Glioma Cells C6

The growth inhibiting and apotosis-inducing effects of aqueous extract of Albizia julibrissin Durazz flower(AJDF) on the rat glioma cells C6 in vitro were studied by MTT assays and flow cytometry methods.The results showed that the water extract of AJDF had obvious suppression on the proliferation of C6 cell lines,which had significant dose-and time-dependent relationships,the LD50 was respectively 305 μg/mL,50 μg/mL,15 μg/mL for 24 h,48 h,72 h.The flow cytometric analysis displayed that 200 μg/mL,300 μg/mL water extract of AJDF can both induce C6 cell lines early phase apotosis,apotosis rate was respectively 4.3%,14.3%,but the blank control group was only 0.7%.The mechanisim of inhibiting proliferation might be related to cell apoptosis.