| 欧亚旋覆花总黄酮对糖基化终末产物诱导L929细胞衰老及RAGE表达的影响 |
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| 引用本文： | 刘露,龙媛媛,郭宗雨,唐维,果磊.欧亚旋覆花总黄酮对糖基化终末产物诱导L929细胞衰老及RAGE表达的影响[J].中草药,2018,49(3):626-632. |
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| 作者姓名： | 刘露 龙媛媛 郭宗雨 唐维 果磊 |
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| 作者单位： | 重庆医科大学附属第一医院 烧伤整形美容外科, 重庆 400016,重庆医科大学附属第一医院 烧伤整形美容外科, 重庆 400016,重庆医科大学附属第一医院 烧伤整形美容外科, 重庆 400016,重庆医科大学附属第一医院 神经外科, 重庆 400016,重庆医科大学附属第一医院 烧伤整形美容外科, 重庆 400016 |
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| 基金项目： | 重庆市卫生局中医药科技资助项目（ZY20132123） |
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| 摘 要： | 目的探讨欧亚旋覆花总黄酮(IBFTF)对糖基化终末产物(AGEs)诱导小鼠皮肤成纤维细胞株L929衰老的保护作用,和AGEs特异性受体(RAGE)的表达机制。方法采用0.100 g/L AGEs培养液刺激离体培养的L929细胞48 h制备衰老模型,药物组分别给予不同质量浓度IBFTF(0.1、0.2、0.4 g/L)继续作用6 h,阳性对照组给予0.1 g/L氨基胍半硫酸盐(AG)继续作用6 h,对照组给予完全培养液。检测胞内β-半乳糖苷酶阳性染色细胞数量和细胞周期比例等衰老指标;检测胞内活性氧(ROS)、超氧化物歧化酶(SOD)和丙二醛(MDA)水平等氧化应激指标。采用细胞免疫荧光检测RAGE荧光强度,Western blotting和q RT-PCR分别检测RAGE蛋白和m RNA表达情况。结果 IBFTF可显著抑制AGEs诱导的L929细胞衰老并降低RAGE的蛋白和m RNA表达水平;不同质量浓度IBFTF作用于衰老的L929细胞后,可显著提高细胞内SOD活性,显著降低MDA和ROS水平,具有浓度依赖性。结论 IBFTF对AGEs诱导衰老的L929细胞有保护作用,其机制可能是通过抑制RAGE表达来实现的。
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| 关 键 词： | 欧亚旋覆花总黄酮 糖基化终末产物 糖基化终末产物特异性受体 衰老 皮肤成纤维细胞 超氧化物歧化酶 丙二醛 活性氧 |
| 收稿时间： | 2017/6/18 0:00:00 |
Inula britannica flower total flavonoids inhibiting expression of RAGE in aging L929 cells induced by advanced glycation end products |
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| LIU Lu,LONG Yuan-yuan,GUO Zong-yu,TANG Wei and GUO Lei.Inula britannica flower total flavonoids inhibiting expression of RAGE in aging L929 cells induced by advanced glycation end products[J].Chinese Traditional and Herbal Drugs,2018,49(3):626-632. |
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| Authors: | LIU Lu LONG Yuan-yuan GUO Zong-yu TANG Wei and GUO Lei |
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| Affiliation: | Department of Burn and Plastic Surgery, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China,Department of Burn and Plastic Surgery, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China,Department of Burn and Plastic Surgery, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China,Department of Neurosurgery, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China and Department of Burn and Plastic Surgery, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China |
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| Abstract: | |
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| Keywords: | Inula britannica flower total flavonoids advanced glycation end products receptor advanced glycation end products aging L929 SOD MDA oxygen species |
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