荧光分析法在针刺镇痛原理研究中的应用

<正> 随着荧光仪器技术的发展,荧光分析方法日趋完善。目前荧光分析方法已广泛地应用到药物分析、临床化验、医学和生物学基础研究等各个领域,分析对象有无机离子和化合物、有机化合物、氨基酸、蛋白质、酶、核酸、糖、维生素和甾族化合物等等。近年来,荧光分析法在针刺镇痛的神经化学原理研究中得到广泛应用。一、荧光分析法的基本原理和特点某些物质被一定波长的紫外光照射,分子吸收能量,由基态跃迁到较高的能级(激发

APPLICATIONS OF FLUOROMETRIC ANALYSIS IN STUDIES ON ACUPUNCTURE ANALGESIA MECHANISM

Spectrophoto-fluorometry is now widely used to assay concentrations of the central monoamine neurotransmitters in brain, cerebrospinal fluid, blood and urine etc. This article reviews the recent main applications of the techenique in the studies on acupuncture analgesia mechanism: (1) cha- nges of contents and turnover rates of the central monoamine neurotra- nsmitters under acupuncture analgesia; (2) qualities (strengthening or we- akening) and sites of actions of the central monoamine neurotransmitters in acupuncture analgesia processes;(3) the interrelationship between the central monoamines and other neurotransmitters in acupuncture analgesia processes. The basic principle and specialities of the fluorometric analysis and the factors effecting the analysis are briefly described in the paper.