突触核蛋白在结直肠癌中的表达模式及其临床意义

目的 探讨结直肠癌组织中突触核蛋白（Synuclein）的表达模式，并分析其与临床病理特征的关系。方法 半定量RT-PCR检测20例结直肠癌及其癌旁组织中α-synuclein、β-synuclein和γ-synuclein基因的mRNA水平，免疫组化SP法检测51例结直肠癌及其癌旁组织中α-synuclein、β-synuclein和γ-synuclein蛋白的表达。结果 结直肠癌组织中α-synuclein、β-synuclein和γ-synuclein mRNA的的相对表达量分别为0.62±0.32、0.61±0.32和0.72±0.37，显著高于癌旁组织的0.41±0.27、0.40±0.25和0.45±0.25（P=0.034，P=0.026，P=0.007）。γ-synuclein蛋白在结直肠癌组织中的阳性表达率为47.1％，显著高于癌旁组织的23.5％（P=0.022），而α-synuclein、β-synuclein蛋白在结直肠癌和癌旁组织中的表达差异无统计学意义。在α-synuclein、β-synuclein和γ-synuclein表达模式的8种组合中，任一种蛋白表达、α-synuclein或γ-synuclein、β-synuclein或γ-synuclein、α-synuclein和γ-synuclein在结直肠癌组织中的表达率明显高于癌旁组织（P＜0.05）。α-synuclein、β-synuclein蛋白的表达与临床病理特征无关，γ-synuclein蛋白的表达与分期和淋巴结转移有关（P=0.047，P=0.048）。淋巴结转移及分期较晚者任一蛋白的阳性表达率明显高于无淋巴结转移及分期较早者（P=0.030，P=0.040）。结论 Synuclein在结直肠癌组织中的表达较癌旁组织有增高的趋势，其中γ-synuclein对于评估结直肠癌病程的发展和转归具有潜在价值。

Expressions of synucleins in colorectal cancer and their clinical significance

Objective To investigate exprsssions of synucleins in colorectal cancer （CRC） tissues and their clinical significance. Methods We measured the mRNA levels of α-synuclein, β-synuclein and γ-synuclein of tumor tissues and adjacent normal tissues in 20 cases by semi-quantitative RT-PCR and their protein expressions in 51 cases by immunohistochemisty. Results The mRNA levels of α-synuclein, β-synuclein and γ-synuclein were 0. 62 ± 0. 32, 0. 61 ± 0. 32 and 0. 72 ± 0. 37 in CRC tissues, significantly higher than 0. 41 ±0. 27, 0. 40 ±0. 25 and 0. 45 ±0. 25 in adjacent normal tissues （P =0. 034, P =0. 026, P =0. 007）. The positive rate of γ-synuclein protein in CRC samples was 47. 1% （24/51）, significantly higher than 23.5% （ 12/51 ） in adjacent normal tissues （ P = 0. 022） , while the expressions of α-synuclein and β-synuclein in CRC and adjacent normal tissues had no difference. In 8 pat- terns of α-synuclein, β-synuclein and γ-synuclein expressions, the positive rates of α-synuclein or γ-synuclein, β-synuclein or γ-symuclein , α-synuclein and γ-synuclein, and any synuclein expression were higher than those in adjacent tissues（ P 〈 0. 05 ）. α-synuclein and β-synuclein had no correlation with clinical features, while γ-synuclein protein expression was significantly correlated with stage and lymph node metastasis of CRC （P = 0. 047, P = 0. 048）. Compared with CRC of late stages and lymph node metastasis, the ex- pression of any synuclein in early stages and without lymph node metastasis was much lower （P = 0. 030, P = 0. 040）. Conclusion α-synuclein, β-synuclein and γ-synuclein are highly expressed in CRC tissues. γ-synuclein protein is valuable for evaluation of CRC progression.