Binding of different antigen-enzyme and antibody-enzyme conjugates by intracellular antibodies in cytoplasm and Golgi complex of plasma cells |
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Authors: | N van Rooijen N Kors E Claassen D M Boorsma |
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Affiliation: | (1) Immunocytochemistry Unit, Faculty of Medicine, Department of Histology, Free University, P.O. Box 7161, NL-1007 MC Amsterdam, The Netherlands;(2) Department of Dermatology, Free University, P.O. Box 7161, NL-1007 MC Amsterdam, The Netherlands |
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Abstract: | Summary Intracellular immunoglobulins in plasma cells were characterized by antigen-enzyme conjugates and anti-immunoglobulin antibody-enzyme conjugates applied in a double immunocytochemical approach. After their assemblage, immunoglobulins in the cytoplasm of anti-TNP antibody producing plasma cells can be demonstrated both by TNP-enzyme conjugates and by anti-immunoglobulin ( or chain specific) antibody-enzyme conjugates. Once arrived in the Golgi complex (GC) detection with TNP-enzyme conjugates remains possible, but anti-immunoglobulin antibody-enzyme conjugates did not bind to a detectable degree. Similar results were obtained in experiments where immunoglobulin-enzyme conjugates were used both as an antigen-enzyme conjugate and as an antibody-enzyme conjugate. |
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