复方鸡骨草颗粒中栀子苷的HPLC含量测定

目的建立高效液相色谱法测定复方鸡骨草颗粒中栀子苷的方法。方法采用反相高效液相色谱法测定。用Shim-pack C18色谱柱(4.6 mm×250 mm,5μm),以乙腈-水(12∶88)为流动相,流速为1.0 mL.min-1,检测波长238 nm,柱温为室温,进样量为10μL。结果栀子苷保留时间约为12 min左右,与其他峰的分离度大于1.5。栀子苷的线性范围为0.15～3.00μg,r=0.999 9,平均加样回收率为99.10%(n=5)。结论该方法简便快速,结果准确可靠,可用于复方鸡骨草颗粒中栀子苷的含量测定,以确保该药的安全、合理使用。

Determination of jasminoidin in Compound Abrus Cantoniensis Particles by HPLC

Objective To establish an HPLC method for determination of jasminoidin in Compound Abrus Cantoniensis Par- ticles. Methods The determination was carried out with RP - HPLC on Shim - pack Cls column （4.6 mm × 250 mm, 5 μm ） . The mobile phase was acetonitrile- water（ 12： 88） and the flow rate was 1.0 mL · min^-l. The detection wavelength was 238 nm at room temperature and the sample volume was 10 μL. Results The retention time of jasmionoidin was about 12 min and the resolution was higher than 1.5. The linear relationship was 0.15- 3.00 μg（ r =0. 999 9 ） and the average recovery was 99.10% （n = 5）. Conclusion This method was simple, rapid and reliable, and it can be used for the determination of jasminoidin in Compound Abrus Cantoniensis in order to ensure the safe and rational use of the drug.