| 三疣梭子蟹肌肉乳化病的同工酶病理变化研究 |
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| 引用本文： | 陈寅儿,王国良,金 珊,李 政.三疣梭子蟹肌肉乳化病的同工酶病理变化研究[J].海洋科学,2008,32(5):36-39. |
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| 作者姓名： | 陈寅儿 王国良 金 珊 李 政 |
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| 作者单位： | 宁波大学,生命科学与生物工程学院,浙江,宁波,315211 |
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| 基金项目： | 浙江省科技厅资助项目
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浙江省自然科学基金 |
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| 摘 要： | 采用垂直平板聚丙烯酰胺凝胶电泳技术(PAGE),对患有肌肉乳化病的三疣梭子蟹(Portunus trituberculatus)3种组织中10种同工酶及肌浆蛋白的酶谱表型进行分析,并就患病后酶谱变化特征及原因进行了讨论。证实在部分组织中,除SOD酶外9种酶及肌浆蛋白的酶带均出现明显的紊乱现象,表明病蟹的病理机制极可能与代谢异常有关,其特异性变化可以作为疾病早期诊断及病理分析的生化辅助指标。
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| 关 键 词： | 三疣梭子蟹(Portunus trituberculatus) 肌肉乳化病 同工酶 |
| 收稿时间： | 1/4/2005 12:00:00 AM |
| 修稿时间： | 2008/1/25 0:00:00 |
Analysis of the myogen and isozymes of the Portunus trituberculatus with emusification disease |
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| CHENG Yin-er,WANG Guo-liang,JIN Shan,LI Zheng.Analysis of the myogen and isozymes of the Portunus trituberculatus with emusification disease[J].Marine Sciences,2008,32(5):36-39. |
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| Authors: | CHENG Yin-er WANG Guo-liang JIN Shan LI Zheng |
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| Abstract: | The myogen(Pm),and 10 kinds of Isozymes in the 3 kinds of tissue of healthy Portunus trituberculatus and of individuais with emusification disease were studied by polyacrylamide gel electrophoresis.The result showed that the electrophoretogram changed greatly in the emusification disease Portunus trituberculatus.Compared with the isozyme patterns of some tissues from healthy crabs,those of diseased ones are different.It is considered that isozymes electrophoretogram can be used as a physiological as well as pathological index in the emusification disease. |
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| Keywords: | Portunus trituberculatus emusification disease isozymes |
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