| 微管蛋白聚合及其与紫杉醇作用的量热学研究 |
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| 引用本文： | 韩公社,阎隆飞,王保怀,张有民.微管蛋白聚合及其与紫杉醇作用的量热学研究[J].物理化学学报,1997,13(11):969-973. |
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| 作者姓名： | 韩公社 阎隆飞 王保怀 张有民 |
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| 作者单位： | College of Biological Science,China Agricultural University,Beijing 100094,Department of Chemistry,Peking University,Beijing 100871 |
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| 基金项目： | 国家自然科学基金!39470348 |
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| 摘 要： | 微管作为细胞骨架的主要成分之一，在细胞内执行许多功能.微管是一个动态的结构，既参与细胞运动和细胞内物质运输［‘个又感受、转导外界信号问的刺激产生相应灵敏的反应.尤其它是许多药物，如：紫杉醇（Taxol）、秋水仙素（Colchicine）、等作用的靶子问，因而，微管已成为筛
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| 关 键 词： | 微管蛋白 紫杉醇 热谱图 |
| 收稿时间： | 1997-08-25 |
| 修稿时间： | 1997-09-22 |
Microcalorimetric Studies of the Tubulin Polymerization and the Interaction between Tubulin and Taxol |
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| Han Gongshe, Yen Lungfei.Microcalorimetric Studies of the Tubulin Polymerization and the Interaction between Tubulin and Taxol[J].Acta Physico-Chimica Sinica,1997,13(11):969-973. |
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| Authors: | Han Gongshe Yen Lungfei |
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| Affiliation: | College of Biological Science,China Agricultural University,Beijing 100094|Department of Chemistry,Peking University,Beijing 100871 |
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| Abstract: | The heat properties of porcine brain tubulin polymerization and the interaction of the tubulin with taxol were studied with an MS-80 Calvet Microcalorimeter .The heat vibration occured within 1.5h when tubulin solution was diluted from a highe r concentration to a lower one. The thermodynamic parameters of tubulin polymerization at 303.15K and 298. 15K were measured in 10μmol?L-1 tubulin solution, its △H's were 22kJ?mol-1 and 29.3kJ?mol-1，respectively.Adding different concentration of taxol in the course of polymerization of tubulin give rise to a great change in △H. The time of taxol combining to polymerized microtubule was about one minute after the beginning of the reaction. The mechanism of tubulin polymerization under different conditions was discussed. |
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| Keywords: | Tubulin Taxol Thermogram |
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