| 长期吸烟对大鼠肺E-选择素和IL-8表达影响 |
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| 引用本文： | 万丹,李文芳,石梦蝶,刘福荣.长期吸烟对大鼠肺E-选择素和IL-8表达影响[J].中国公共卫生,2011,27(8). |
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| 作者姓名： | 万丹 李文芳 石梦蝶 刘福荣 |
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| 作者单位： | 武汉科技大学黄家湖校区医学院预防医学系,湖北,武汉,430065 |
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| 摘 要： | 目的探讨长期吸烟对大鼠的肺损伤及相关炎性因子表达的影响。方法 56只雄性SD大鼠,随机分为对照组和低、中、高3个剂量吸烟组,采用自主开发的吸烟机给烟12周;放免法检测支气管肺灌洗液(BALF)中白介素-8(IL-8)的含量,免疫组化法检测肺血管内皮细胞E-选择素的表达水平。结果高、中、低剂量吸烟组大鼠BALF中IL-8的含量分别为(0.77±0.010)、(0.71±0.171)、(0.62±0.088)ng/mL,高于对照组(0.28±0.133))ng/mL,差异有统计学意义(P<0.01);高、中、低剂量吸烟组E-选择素的表达分别为(0.27±0.030)、(0.18±0.034)、(0.16±0.025),均高于对照组(0.07±0.023),差异有统计学意义(P<0.01);中剂量吸烟组肺血管内皮细胞E-选择素与BALF中IL-8的含量呈明显正相关(r=0.716,P<0.01)。结论长期吸烟导致气道炎症介质IL-8和E-选择素的表达升高,促进气道内炎症反应,对支气管肺组织造成严重损害。
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| 关 键 词： | 吸烟 E-选择素 白介素-8(IL-8) 炎症 |
Effects of long-term smoking on expression of IL-8 and E-selectin in rat lungs |
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| WAN Dan,LI Wen-fang,SHI Meng-die,et al..Effects of long-term smoking on expression of IL-8 and E-selectin in rat lungs[J].Chinese Journal of Public Health,2011,27(8). |
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| Authors: | WAN Dan LI Wen-fang SHI Meng-die |
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| Affiliation: | WAN Dan,LI Wen-fang,SHI Meng-die,et al.Departement of Preventive Medicine,Medical College,Wuhan University of Science and Technology(Wuhan 430065,China) |
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| Abstract: | Objective To investigate the influence of long-term smoking on the expression of inflammatory factors in rat lung tissue.Methods Fifty-six male SD rats were randomly divided into four groups:control,low-,moderate-,and high-dose group.The rats in each group were exposed to cigarette smoke at different dose for 12 weeks.The level of interleukin-8(IL-8) in bronchoalveolar lavage fluid(BALF) was measured with radioimmunoassay.Immunohistochemistry assay was carried out to examine the expression of E-selectin in ... |
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| Keywords: | smoking E-selectin IL-8 inflammation |
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