JNK/c-Jun信号通路在大鼠胸主动脉瘤模型中的表达及意义

目的 探讨c -Jun氨基末端激酶(JNK)及c-Jun在实验性大鼠胸主动脉瘤中的表达及其意义. 方法 将20只成年雄性Wistar大鼠随机分为两组,即对照组和手术组.采用氯化钙(CaCl2)诱导法制备胸主动脉瘤模型,于术后4周取外敷CaCl2段胸主动脉.用免疫组织化学和Western blotting方法检测动脉瘤壁JNK和c-Jun的表达. 结果 免疫组织化学显示,磷酸化JNK(p-JNK)和磷酸化c-Jun( p-c-Jun)主要表达于动脉瘤中膜,而对照组表达很弱.Western blotting显示,动脉瘤组织中p-JNK和p-c-Jun表达均强于对照组. 结论 p-JNK和p-c-Jun在动脉瘤中表达强于对照组,JNK/c-Jun信号通路可能在动脉瘤形成过程中起重要作用.

Expression and significance of JNK/c-Jun pathway in rat thoracic aortic aneurysm

Objective To investigate the expression and significance of c-Jun N-terminal kinase (JNK) and c-Jun in the experimental rat thoracic aortic aneurysm. BR>Methods Twenty adult male Wistar rats were randomly divided into two groups: sham group and operated group. The thoracic aortic aneurysm models were induced by CaCl2 The thoracic aortic segments were collected at the 4th week after surgery. Immunohistochemistry and Western blotting were performed to detect the expression of JNK and c-Jun in the wall of the aneurysm. Results Immunohistochemical staining results showed that p-JNK and p-c-Jun were located extensively in smooth muscle cells of the tunica media in the aortic aneurysm, while there was only a little positive staining in the control group. Western blotting results indicated that the expression levels of p-JNK and p-c-Jun in the aortic aneurysm were higher than those in the control. Conclusion The expressions of p-JNK and p-c-Jun in thoracic aortic aneurysm are stronger than that in the control. The JNK/c-Jun pathway may play a significant role in the development of aortic aneurysm. BR>