维生素K_1治疗毛细支气管炎的实验研究

以NO_2染毒复制动物毛细支气管炎模型以维生素K_1、氨茶碱等进行实验治疗观察,以维生素K_1治疗组的疗效最佳。

TREATMENT OF ACUTE BRONCHIOLITIS IN RATS WITH VITAMINE K_1

In order to reproduce acute bronchiolitis studies on exposure to No2 have been undertaken in the last decades. This paper deals with No2 induced brnchopathy in rats, and its treatment with vitamine K1, aminophyl-line or 10% glucose etc. One hundred and eighty male rats, weighing 111.7g each, were used in our experiment. The rats were exposed to No2 for 4hr, the concentration of which was 250-450 ppm. The rats were divided into 6 groups. Group 1, which was for control, neither inhaled No2 nor under- went treatment. Groups 2-6 inhaled No2 for 4 hr. Group 2 were killed soon after the inhation. Groups 3-6 were treated with vitamine K1 (Group 3 and Group 4), aminophylline (Group 5) or 10% glucose (Group 6). Under the optical microscope in the lungs of rats of Group 1 (the control group) no anatomic abnormality was obsered in the bronchioles (Figl). Group 2 showed many different pathologic features as follows: (1) Proliferation of the epithelium. It was likely that there were smooth muscle spasms, lysis and desqua-mation of epithelial cells (Fig 1); (2) Lymphocytes invaded the peribron-chial tissue and migrated into the area between epithelial cells (Fig2); (3) The partial or complete obstrution (Fig2) might result from edema of sub-muscosa, dense plugs that were composed of alveoler debris and strands of fibrin that often were formed from the small bronchii and bronchioles; (4) Capillary abnormality in varying degrees (Fig3); (5) An associated pathy interstitial pneumonia might occur with the thicking of the intervalyular walls, but it was vere rare. When Group 3 was compared with groups 4,5,6, it was found that the former, viz vitamine K1 group, showed better curative results. Aminophylline (group 5) was proved to have no effect on rat-bronchiolitis, because the lesion involved edema and nflammation of bronchialar epithelium, 10% glucose was of little therapeutic value. However, vitamine K1 has been found to be helpful in improvimg smooth muscle spasms or bleeding in rat-bronchiolitis.