| 光谱法研究甲氨喋呤与牛血清白蛋白的相互作用 |
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| 引用本文： | 谢文俊,冯玉萍,曹胜利,赵玉芬.光谱法研究甲氨喋呤与牛血清白蛋白的相互作用[J].光谱学与光谱分析,2006,26(10):1876-1879. |
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| 作者姓名： | 谢文俊 冯玉萍 曹胜利 赵玉芬 |
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| 作者单位： | 1. 清华大学化学系，生命有机磷化学及化学生物学教育部重点实验室, 北京 100084
2. 首都师范大学化学系, 北京 100037 |
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| 摘 要： | 用荧光和紫外吸收光谱法，研究了抗癌药物甲氨喋呤(MTX)与牛血清白蛋白(BSA)的相互作用。结果表明：在生理条件下，甲氨喋呤对牛血清白蛋白荧光有较强的猝灭作用，其猝灭方式为静态猝灭。根据猝灭结果，求出了不同温度下反应的结合常数及反应热力学参数，并据此确定了它们相互作用的主要形式。
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| 关 键 词： | 甲氨喋呤 牛血清白蛋白 荧光光谱 紫外光谱 相互作用 |
| 文章编号： | 1000-0593（2006）10-1876-04 |
| 收稿时间： | 2005-06-28 |
| 修稿时间： | 2005-11-08 |
Study of the Interaction between Methotrexate and Bovine Serum Albumin by Spectrometry |
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| XIE Wen-jun,FENG Yu-ping,CAO Sheng-li,ZHAO Yu-fen.Study of the Interaction between Methotrexate and Bovine Serum Albumin by Spectrometry[J].Spectroscopy and Spectral Analysis,2006,26(10):1876-1879. |
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| Authors: | XIE Wen-jun FENG Yu-ping CAO Sheng-li ZHAO Yu-fen |
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| Affiliation: | 1. Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology(Ministry of Education), Department of Chemistry, Tsinghua University, Beijing 100084, China2. Department of Chemistry, Capital Normal University, Beijing 100037, China |
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| Abstract: | The interaction of methotrexate(MTX) and bovine serum albumin(BSA) was studied by fluorescence and absorption spectra.The results show that methotrexate strongly quenched the fluorescence of bovine serum albumin,and the quenching mechanism is a static quenching procedure.The binding constants and the number of binding sites between MTX and BSA at different temperatures were calculated.Furthermore,the enthalpy and entropy changes in the interaction were also obtained,and the primary binding pattern between MTX and BSA was interpreted as hydrophobic interaction. |
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| Keywords: | Methotrexate(MTX) Bovine serum albumin(BSA) Fluorescence spectra Absorption spectra Interaction |
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