Allele-specific PCR reveals thatCYP6D1 is on chromosome 1 in the house fly,Musca domestica |
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| Authors: | N Liu T Tomita J G Scott |
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| Affiliation: | (1) Department of Entomology, Cornell University, Comstock Hall, 14853-0901 Ithaca, New York, USA;(2) Present address: Department of Medical Entomology, National Institute of Health, 1-23-1, Toyama, Shinjuku-ku, 162 Tokyo, (Japan) |
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| Abstract: | A cytochrome P450, termed P450lpr, is the major P450 responsible for pyrethroid resistance in the Learn-PyR (LPR) strain of house fly. Recently, the putative gene (CYP6D1) coding for P450lpr has been sequenced from the LPR and aabys strains of house fly. Allele-specific polymerase chain reaction (ASPCR) was used for linkage group analysis with backcross progeny from the wild type LPR strain and a multiple marker strain (aabys). We found thatCYP6D1 is linked to chromosome 1. The possible role of regulatory or modifying genes responsible for elevated P450lpr expression is discussed in relation to the chromosomal linkage ofCYP6D1. |
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| Keywords: | Cytochrome P450 monooxygenase Insecta insecticide resistance |
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