拟南芥温敏雄性不育突变体atms1的获得及表型分析

从甲基磺酸乙酯（ethylmethane sulfonate,EMS）化学诱变建立的野生型拟南芥（Col-0）突变体文库中筛选到1株突变体.在低温16 ℃时，该突变体的雄性育性与野生型没有显著差异，花粉染色呈现100%可育.随着培养环境温度的升高，突变体花粉育性逐渐下降，因此，该突变体为一温敏雄性不育突变体,并被命名为atms1（ambient temperature-sensory male sterility 1，即环境温度敏感雄性不育1）.花药切片结果显示，在23 ℃以下，该突变体花药各个发育时期的形态与野生型花药没有显著的差异；而27 ℃处理1周后的突变体花药呈现多种表型：同一朵花中各个花药的发育时期出现显著分化，花粉母细胞胼胝体单薄，绒毡层发育滞后于同时期的野生型花药.遗传分析确定，atms1的不育表型是由单个隐性核基因控制的.

Thermo-Sensitive Male Sterile Mutant atms1 of Arabidopsis thaliana Access and Phenotypic Analysis

An ambient temperature Arabidopsis thaliana male sterile mutant ambient temperature-sensory male sterile 1(atms1),generated from ethylmethane sulfonate(EMS) mutagenesis has been identified.At low temperature 16 ℃,atms1 showed 100% pollen viability and silique fertility,while with the culture temperature increased,atms1 showed decreasing pollen viability and silique fertility.Therefore atms1 is a temperature sensory male sterile mutant.The morphological assay demonstrated that atms1 anther showed few significant impairments compared with wild-type anther when ambient temperature was below 23 ℃.At higher temperature 27 ℃,the atms1 anther exhibited pleiotropic phenotypes: the developmental phases of anthers within one flower differed significantly;the callose layer of pollen mother cell of mutant was much thinner than that of wild-type,and the atms1 tapetum development exhibited much retarded than corresponding wild-type tapetum.Genetic assay demonstrated that the phenotypes of atms1 were controlled by a single recessive locus.