汽爆玉米秸秆渣诱导产纤维素酶及其水解特性

为提高玉米秸秆的生物降解效率，研究了汽爆处理玉米秸秆渣对纤维素酶的诱导作用及所产纤维素酶的水解特性。结果表明：玉米秸秆采用汽爆处理可以去除大量的半纤维素成分，获得的汽爆渣对里氏木霉NL02合成纤维素酶的诱导作用良好，以10 g/L的汽爆渣为碳源发酵7 d滤纸酶活达到1.90 U /mL。发酵5 d获得的汽爆酶水解效果最佳，当酶用量25 U/g，底物质量浓度50 g/L条件下，水解24 h酶解得率达到95.9%，水解48 h葡萄糖产率为76.4%。当汽爆渣质量浓度为80 g/L，酶用量为15 U/g时，48 h酶解效率达到90.1%，葡萄糖产率为62.9%。

Characteristics of cellulase inducement and hydrolysis by steam exploded corn stover

The characteristics of cellulase inducement and hydrolysis by steam exploded corn stover were investigated.Steam explosion could remove hemicellulose from corn stover and the acquired residue was a good inducer for cellulase biosynthesis using Trichoderma reesei NL02.When the concentration of substrate was 10 g/L,the filter paper activity reached 1.90 U/mL after cultivation for seven days.The four produced and the two other enzymes were characterized by various enzyme activity measurements and steam explode...