儿茶素对LoVo细胞生长周期的影响和诱导凋亡的作用

目的 探讨表没食子儿茶素没食子酸酯（ＥＧＣＧ）、表没食子儿茶素（ＥＧＣ）、表儿茶素没食子酸酯（ＥＣＧ）和表儿茶素（ＥＣ）对大肠癌ＬｏＶｏ细胞生长周期的影响和它们诱导ＬｏＶｏ细胞凋亡作用及其差异性。方法 用ＭＴＴ法、琼脂糖凝胶电泳、透射电镜和流式细胞术观察４种儿茶素处理ＬｏＶｏ细胞后,它们对ＬｏＶｏ细胞的生长抑制作用、细胞生长周期的改变以及诱导其凋亡形态学和生化方面的改变。结果 ＥＧＣＧ和ＥＧＣ对Ｌ

Effects of different catechins on cell cycle arrest and induction of apoptosis in LoVo cells

Objective To investigate the effects of green tea (-) epigallocatechin 3 gallate (EGCG), (-) epigallocatechin (EGC), (-) epicatechin 3 gallate(ECG)and (-) epicatechin (EC) on the cell cycle arrest and induction of apoptosis in human colon carcinoma LoVo cell line. Methods The cytotoxicity, cell cycle arrest and apoptosis associated alterations in morphology and biochemistry were examined using the tetrazolium salt reduction (MTT) assay, gel electrophoresis, transmission electron microscope and flow cytometry after treatment of LoVo cells with the four catechins. Results Treatment of LoVo cells with EGCG and EGC resulted in the growth suppression and induction of apoptosis in a time and concentration dependent manner. ECG and EC, however, did not have the same effects. In addition, the four catechins had different effects on the cell cycle of LoVo cells. Treatment with EGCG, EGC and ECG caused LoVo cells arrest at G1 phase in the cell cycle progression, whereas EC resulted in an arrest at S phase. Conclusions The cytotoxicity of the four catechins to LoVo cells is significantly different. The difference is associated with their effects on cell cycle and induction of apoptosis in LoVo cells.