| 枯草芽孢杆菌谷氨酰胺转氨酶的异源表达 |
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| 引用本文： | 刘凯,刘逸寒,张艳,田耀,路福平.枯草芽孢杆菌谷氨酰胺转氨酶的异源表达[J].天津科技大学学报,2012,27(3):1-5. |
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| 作者姓名： | 刘凯 刘逸寒 张艳 田耀 路福平 |
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| 作者单位： | 工业发酵微生物教育部重点实验室,天津科技大学生物工程学院,天津300457 |
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| 基金项目： | “863"“十二五”农村领域国家科技计划课题资助，国家自然科学基金资助项目 |
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| 摘 要： | 以野生型枯草芽孢杆菌基因组DNA为模板,PCR扩增获得带有SD序列及谷氨酸棒状杆菌信号肽ΔS0949的BTG基因.将其与大肠杆菌-谷氨酸棒状杆菌穿梭表达载体pXMJ19连接,构建重组质粒pXMJ19-Sbtg转化谷氨酸棒状杆菌ATCC13032.经IPTG诱导后该重组菌发酵液具有交联酪蛋白的能力,表明该重组菌能够实现分泌表达.
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| 关 键 词： | 谷氨酰胺转氨酶 谷氨酸棒状杆菌 信号肽 分泌表达 |
Heterogeneous Expression of Bacillus subtilis Transglutaminase |
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| LIU Kai
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LIU Yihan
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ZHANG Yan
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TIAN Yao
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LU Fuping.Heterogeneous Expression of Bacillus subtilis Transglutaminase[J].Journal of Tianjin University of Science & Technology,2012,27(3):1-5. |
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| Authors: | LIU Kai LIU Yihan ZHANG Yan TIAN Yao LU Fuping |
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| Affiliation: | (Key Laboratory of Industrial Fermentation Microbiology,Ministry of Education,College of Biotechnology,Tianjin University of Science & Technology,Tianjin 300457,China) |
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| Abstract: | Gene Sbtg with SD sequence and signal peptide ΔS0949 Corynebacterium glutamicum was obtained from the genomic DNA of wild type Bacillus subtilis though PCR amplification.It was then inserted into an E.coli-C.glutamicum shuttle vector pXMJ19 to construct expression plasmid pXMJ19-Sbtg.The recombined plasmid was then transformed into Corynebacterium glutamicum ATCC13032.Induced by IPTG,the fermentatiom broth had a cross-linking ability.The result indicated that gene Sbtg was a secretion expression. |
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| Keywords: | transglutaminase Corynebacterium glutamicum signal peptide secretion expression |
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