番茄粉红色果实相关基因的dCAPS和InDel标记开发与应用

采用SuperBSA方法分别构建100份粉红色番茄材料和100份红色番茄材料的DNA混合池,对测序结果的SNP位点进行分析。结果表明:在1号染色体上的127个SNP位点中,有6个与番茄果实颜色高度相关,其中1个位点与粉红色果实高度相关。利用该SNP位点开发了1个共显性d CAPS标记,在红色、粉红色和杂合F_13种基因型之间的多态性较好。同时,利用番茄重测序数据分析番茄起源时发现,在粉红色番茄中SIMYB12基因的上游存在1个603bp的缺失,利用该缺失突变位点开发了1个共显性In Del标记,在红色、粉红色和杂合F_13种基因型之间也表现出较好的多态性。利用新开发的2个标记对F_2群体进行遗传分析,鉴定结果与田间表型观察结果符合度均达到95%以上,表明这2个标记可以用于番茄苗期分子标记辅助选择。

Development and Application of dCAPS and InDel Markers in Pink Tomato Fruitrelated Genes

Super BSA was performed to screen SNP between 2 mixed DNA pools，which contained 100 pink tomatoes and 100 red tomatoes．SNPs of the tested result were analyzed．The result indicated that 6 SNPs among 127 SNPs on chromosome 1 were highly correlated with the color of tomato fruit，and 1 SNP was closely linked to pink tomato fruit．By this SNP，a polymorphism dCAPS was developed and showed well correlation with red，pink and heterozygous genotypes．It is recently reported that a 603 bp deletion was characterized at the upstream of SIMYB12 gene in pink tomato．Using this deletion，a InDel marker was developed and also showed good polymorphism among red，pink and heterozygous genotypes．These 2 newly developed molecular markerswere further used to analyze genetically the F₂ population．The result was in line with the field observation．Their accordance rate was above 95%，which suggested that these 2 markers can be used for molecular marker assisted selection in tomato breeding．