用高效液相法研究不同龋敏感人群的唾液蛋白成分

为探讨高效疏水色谱对人腮腺唾液蛋白的分离效果，并利用其分析唾液蛋白与龋病发生的相关性，作者采用高效疏水色谱分离、分析了不同个体及不同龋敏感人群的腮腺唾液蛋白，并做了蛋白定性分析。结果表明，疏水色谱可分出６种腮腺唾液蛋白，其中４种蛋白在所有个体的疏水色谱图上出现。定性分析表明，其中３种为不同表现型的富含脯氨酸蛋白（ｐｒｏｌｉｎｅ－ｒｉｃｈｐｒｏｔｅｉｎｓ，ＰＲＰＳ），另外１种为唾液α－淀粉酶。对比分析１５名无龋者及１０名高龋者的疏水色谱图，发现二组人群的蛋白组成类型无明显差异。３种不同表现型的ＰＲＰＳ与龋病间有不同的相关性，它们在二组间相对比有明显差异。提示蛋白比例失调可能为龋易患性的一个重要因素。

A study of parotid salivary proteins from caries free and caries active people by high performance li quid chromatography

In this study,we isolated and analysed parotid salivary proteins of different individuals with different caries susceptibility.Protein fractions isolated were identified by biochemical methods.The results showed that six proteins were isolated from parotid saliva by HIC(hydrophobic interaction chromatography).Four proteins were found in all hydrophobic interaction chromatography.Three of them were identified as different polymorphic forms of proline rich proteins (PRPS) and the other as alpha amylase.Comparing chromatography of fifteen healthy caries free adults with ten caries active adults, we found that there was no difference in salivary protein composition.Three different phenotypes of PRPS had different relations with caries susceptibility and relative ratios of the three PRPS were different between two groups.It suggested that abnormal proportions of salivary proteins may be another important determinant in caries susceptibility.