表达猪繁殖与呼吸综合征病毒ORF5/ORF6基因重组鸡痘病毒的构建与小鼠免疫试验

本试验构建了1株表达猪繁殖与呼吸综合征病毒(Porcine reproductive and respiratory syndrome virus,PRRSV)RF5/ORF6基因的重组鸡痘病毒,并进行了小鼠免疫实验,对其诱导BALB/c小鼠产生体液免疫和细胞免疫反应的能力进行了评价。结果表明,重组鸡痘病毒能够刺激免疫鼠产生特异性PRRSV ELISA抗体,促进特异性T淋巴细胞增殖。对免疫小鼠血清中细胞因子检测结果表明,IFN-γ的分泌显著提高。结果表明,所构建重组鸡痘病毒可以对小鼠起到良好的免疫效果,具有成为抗PRRSV感染新型疫苗的潜力。

Immune responses of mice inoculated with a recombinant fowlpox virus expressing ORF5/ORF6 of porcine reproductive and respiratory syndrome virus

Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most important viral pathogens of swine,and has caused significant economic losses of the pork industry. In this study,A recombinant fowlpox viru-ses containing the ORF5/ORF6 cDNAs of PRRSV(strain Chang Chun) was constructed and evaluated for its abili-ties to induce humoral and cellular responses in mice. All animals vaccinated with rFPV-ORF5-ORF6 developed spe-cific anti-PRRSV ELISA antibody,as well as T-lymphocyte proliferation response. IL-2 and IFN-γ production against PRRSV antigens was detected in rFPV-inoculated mice. The results demonstrated the potential use of a fowlpox vi-rus-based recombinant vaccine in the.control and prevention of PRRSV infections.