Rapid screening of tetrodotoxin in urine and plasma of patients with puffer fish poisoning by HPLC with creatinine correction |
| |
| Authors: | Chung-Him Yu Chun-Fai Yu Sidney Tam Peter Hoi-Fu Yu |
| |
| Affiliation: | 1. Environmental Biotechnology Laboratory, Department of Applied Biology and Chemical Technology , The Hong Kong Polytechnic University , Hong Kong, China vincentyu@ymail.com;3. Environmental Biotechnology Laboratory, Department of Applied Biology and Chemical Technology , The Hong Kong Polytechnic University , Hong Kong, China;4. Division of Clinical Biochemistry, Queen Mary Hospital , The University of Hong Kong , Hong Kong, China |
| |
| Abstract: | A rapid and simple detection method for tetrodotoxin (TTX) in urine and plasma of patients with puffer fish poisoning was developed using commercially pre-packed solid-phase extraction (SPE) cartridges (C18 and weak cation exchange columns) and subsequent analyses by HPLC with UV detection. The detection limit of the standard TTX, TTX-spiked urine and plasma samples were all 10 ng/ml and the average TTX recovery in urine and plasma samples after SPE were 90.3 ± 4.0 and 87.1 ± 2.9%, respectively. It was noticed that the creatinine-adjusted urinary TTX levels obtained within the first 24 h of presentation apparently correlated much better with the severity of poisoning than the urinary TTX concentration without adjusting for variations in concomitant creatinine excretion. |
| |
| Keywords: | chromatography HPLC clean-up SPME toxicology natural toxicants fish and fish products seafood |
|
|
