成年大鼠神经元和星形胶质细胞细胞周期蛋白依赖性激酶抑制因子表达的差异研究

目的比较研究成年大鼠细胞周期蛋白依赖性激酶抑制因子在神经元和星形胶质细胞的表达差异。方法应用免疫荧光和激光扫描共聚焦显微镜观察成年大鼠生理状态下大脑皮层或海马CA1、CA3、DG区神经元和星形胶质细胞细胞周期蛋白依赖性激酶抑制因子(CDKI)p15Ink4b、p21cipl的表达。结果成年大鼠海马区和大脑皮层的神经元有p15Ink4b和p21cipl的表达,细胞核和细胞浆均有表达,且以胞核为主;星形胶质细胞也有上述细胞周期调控蛋白的表达,便细胞数目较少,并且表达这些指标的星形胶质细胞多聚集在海马区。结论成年大鼠大脑皮层和海马区的神经元和星形胶质细胞均表达p15Ink4b和p21cipl,而其在神经元的表达较星形胶质细胞更为普遍。

The difference of CDKIs on astrocytes and neurons in adult rats

Objective To study the difference of CDKIs between astrocytes and neurons in adult rats.Methods The expressions of p15Ink4b and p21ciplof astrocytes and neurons in adult rat cerebral cortex or hippocampus were detected by immunofluorescence and confocal microscope.Results p15Ink4b and p21cipl were expressed on nuclear or/and cytoplasm of postmitotic neurons, mainly on nuclear, of cerebral cortex or hippocampus; Whereas only a few astrocytes expressed CDKIs in adult rat hippocampus and cerebral cortex and most of these astrocytes were located on hippocampal region.Conclusions Both astrocytes and neurons in adult rat cerebral cortex and hippocampus express p15Ink4b and p21cipl which more CDKIs in neuron were expressed p15Ink4b and p21cipl in adult rats hippocampus and cerebral cortex. Compared with that of the astrocytes. the expressions of CDKIs on mature neurons were remarkable.