| 菠菜叶片蔗糖磷酸合成酶的纯化 |
| |
| 引用本文： | 吴勇,苏丽英. 菠菜叶片蔗糖磷酸合成酶的纯化[J]. 植物生理与分子生物学学报, 1989, 15(2): 185-189 |
| |
| 作者姓名： | 吴勇 苏丽英 |
| |
| 作者单位： | 中国科学院上海植物生理研究所，中国科学院上海植物生理研究所，中国科学院上海植物生理研究所，中国科学院上海植物生理研究所 上海 200032 四川大学生物工程研究所，上海 200032，上海 200032，上海 200032 |
| |
| 摘 要： | 经硫酸铵分部沉淀,DEAE-纤维素(DE 52),Sepharose 6B和 AH—4B连续三次柱层析,得到纯化88倍电泳均一的菠菜叶片蔗糖磷酸合成酶。电泳分析该酶分子量为490 kD,是由八个分子量为60 kD的相同亚基组成的寡聚体,等电点为PI=4.l,其最适pH值为6.9。
|
| 关 键 词： | 菠菜叶片 蔗糖磷酸合成酶 纯化 |
The Purification of Spinach Leaf Sucrose Phosphate Synthetase |
| |
| WU Yong,SU Li-Ying,YU Xin-Jian and XIA Shu-Fang. The Purification of Spinach Leaf Sucrose Phosphate Synthetase[J]. Journal Of Plant Physiology and Molecular Biology, 1989, 15(2): 185-189 |
| |
| Authors: | WU Yong SU Li-Ying YU Xin-Jian XIA Shu-Fang |
| |
| Affiliation: | WU Yong,SU Li-Ying,YU Xin-Jian and XIA Shu-Fang Shanghai Institute of Plant Physiology,Academia Sinica,Shanghai 200032 |
| |
| Abstract: | Sucrose phosphate synthetase (EG 2.4.1.14)from spinach leaves has been purified to 88 fold(Table 1) by ammonium sulfate fractiona-tion and column chromatography of DEAE-cellulose, Sepharose 6B and AH-Sepharose-4B(Fig. 2). The enzyme preparation obtained wasshown to be homogeneous as examined by gra-dient polyacrylamide gel electrophoresis (Fig. 3).The enzyme have an approximately molecularweight 490 kD (Fig. 4). SDS-polyacrylamidegel electrophoresis shown that this enzymeconsists of eight identical subunits, each havea molecular weight 60 kD (Fig. 5, 6). Thepurified enzyme had a pH optimum atpH 6. 9 (Fig. 8) and the isoelectric point at4. 1 (Fig. 7). |
| |
| Keywords: | spinack leaf sucrose phosphate synthetase purification |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
