硫辛酸对细胞DNA损伤保护作用的初步研究

目的:用单细胞凝胶电泳技术(SCGE)研究不同浓度过氧化氢(H2O2)分别作用于HepG2细胞2 h后DNA损伤的剂量效应关系,同时观察硫辛酸对H2O2损伤细胞DNA的保护作用. 方法: 将细胞分为对照组、损伤组和保护组,依次加入不同浓度的H2O2及硫辛酸,应用SCGE检测H2O2引起细胞DNA损伤. 结果: 随着H2O2浓度的增加,细胞DNA的损伤程度加重,能使尾长、尾部DNA百分含量和尾距显著增加(P《0.05). 硫辛酸能使H2O2损伤细胞的尾长、尾部DNA百分含量和尾距均较对照组减少(P《0.05). 结论: H2O2对细胞DNA损伤具有剂量效应关系,硫辛酸可以有效地减少H2O2引起的细胞DNA的断裂损伤.

Preliminary investigation on protective function of lipoic acid from DNA damage

AIM: To the study dose-effect relationship between the DNA damage of HepG_2 cells and the effect of different concentrations of hydrogen peroxide (H_2O_2) for 2 h respectively, and to observe the protective function of lipoic acid from the DNA damage of cells at the same time. METHODS: HepG_2 cells were divided into control group, damage group and protective group, then were added with different concentrations of H_2O_2 and lipoic acid. Two hours later, the single cell gel electrophoresis (SCGE) was used to detect the DNA damage. RESULTS: The DNA damage of cells was more serious with H_2O_2 concentration increasing. The tail length, the tail DNA content and the tail moment significantly increased (P<0.05). Lipoic acid could significantly decrease the tail length, the tail DNA content and the tail moment of damaged cells (P<0.05). CONCLUSION: H_2O_2 causes DNA damage of cells in a dose-effect manner, while lipoic acid can reduce effectively this DNA damage caused by H_2O_2.