| Minocycline protects the apoptosis of PC12 cells induced by 1-methyl-4-phenylpyridinium |
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| 引用本文： | Wei SHEN,Shenggang SUN,Xuebing CAO. Minocycline protects the apoptosis of PC12 cells induced by 1-methyl-4-phenylpyridinium[J]. 南京医科大学学报(自然科学版), 2005, 19(5): 247-250 |
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| 作者姓名： | Wei SHEN Shenggang SUN Xuebing CAO |
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| 作者单位： | [1]Department of Neurology, the affiliated Union Hospital of Tongji Medical Collage, Huazhong University of Science and Technology, Wuhan 430022. China |
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| 摘 要： | Objective： To explore the protective effect of minocycline on the apoptosis of cellular parkinsonism models induced by MPP^＋ . Methods： Using PC12 cells as the apoptotic model of dopaminergic neurons, MC and MPP^＋ were added into the culture medium of PC12 cells, and using MTr to assay the cell viability and metabolic state; The cells apoptosis was assayed by electrophoresis method and using flow cytometry FACS to assay the apoptosis ratio. Results： Added the MPP^＋ to get the concentration of 10μmol/L, the cellular parkinsonism model of apoptosis had been prepared. The pre-treatment of MC （ 100/μmol/L） could significantly increase the PC12 cell viability. The apoptosis ratio of MC＋MPP^＋ group was significantly lower than that of MPP^＋ group, but was still significantly higher than that of control group. Conclusion： MC may protect the cell apoptosis induced by MPP^＋ to some extent.
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| 关 键 词： | 二甲胺四环素 PC12细胞 1-甲基-4-苯基吡啶 帕金森病 |
| 收稿时间： | 2005-04-25 |
Minocycline protects the apoptosis of PC12 cells induced by 1-methyl-4-phenylpyridinium |
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| Wei SHEN,Shenggang SUN,Xuebing CAO. Minocycline protects the apoptosis of PC12 cells induced by 1-methyl-4-phenylpyridinium[J]. Acta Universitatis Medicinalis Nanjing, 2005, 19(5): 247-250 |
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| Authors: | Wei SHEN Shenggang SUN Xuebing CAO |
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| Abstract: | Objective: To explore the protective effect of minocycline on the apoptosis of cellular parkinsonism models induced by MPP+ . Methods: Using PC12 cells as the apoptotic model of dopaminergic neurons, MC and MPP+ were added into the culture medium of PC12 cells, and using MTT to assay the cell viability and metabolic state; The cells apoptosis was assayed by electrophoresis method and using flow cytometry FACS to assay the apoptosis ratio. Results: Added the MPP+ to get the concentration of 10μmol/L, the cellular parkinsonism model of apoptosis had been prepared. The pre-treatment of MC (100 μmol/L) could significantly increase the PC12 cell viability. The apoptosis ratio of MC + MPP+ group was significantly lower than that of MPP+ group, but was still significantly higher than that of control group. Conclusion: MC may protect the cell apoptosis induced by MPP+ to some extent. |
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| Keywords: | minocycline PC12 cell apoptosis parkinson disease 1-methyl-4-mhenylpyridinium |
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