| 高效薄层色谱扫描法测定烟草中β-胡萝卜素 |
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| 引用本文： | 时亮,朱晓兰,刘百战,高芸. 高效薄层色谱扫描法测定烟草中β-胡萝卜素[J]. 色谱, 1999, 17(6): 606-606 |
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| 作者姓名： | 时亮 朱晓兰 刘百战 高芸 |
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| 作者单位： | 合肥经济技术学院!安徽合肥230052 |
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| 摘 要： | 摘要：建立了测定烟草中β-胡萝卜素的高效薄层色谱扫描方法，以V（苯）:V（丙酮）=4:4为展开剂，检测波长450nm，线性范围50ng～0.5μg，最低检测限20ng。方法简便、灵敏、快速、重现性好。
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| 关 键 词： | 高效薄层色谱 胡萝卜 烟草 测定 |
Determination of beta-carotene in tobacco by HPTLC] |
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| SHI Liang,ZHU Xiao|lan,LIU Bai|zhan,GAO Yun. Determination of beta-carotene in tobacco by HPTLC][J]. Chinese journal of chromatography, 1999, 17(6): 606-606 |
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| Authors: | SHI Liang ZHU Xiao|lan LIU Bai|zhan GAO Yun |
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| Affiliation: | Hefei Institute of Economics and Technology, Hefei 230052, China. |
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| Abstract: | This paper reports the high performance thin-layer chromatography (HPTLC) determination of beta-carotene in tobacco. The developer used was V(benzene):V(acetone) = 4:1. The detection wavelength was 450 nm. The linear range and the detectable limit of the method were 50 ng-0.5 microgram and 20 ng per spot of beta-carotene respectively. The method has been proved to be simple, sensitive, accurate and reproducible. |
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