HMGB1及TLR2、TLR4在类风湿关节炎中的表达及意义

 目的 探讨HMGB1及TLR2、TLR4在类风湿关节炎患者中的表达。方法 选取活动期类风湿关节炎患者29例，非活动期20例及对照者18例。用酶联免疫吸附试验（ELISA）检测血清HMGB1表达，用多色免疫荧光流式细胞术（FCM）检测外周血单核细胞表面TLR2和TLR4的表达率和蛋白相对含量。结果 活动期RA患者血清HMGB1含量和外周血CD14+单核细胞表面TLR2、TLR4的表达率及蛋白相对含量明显高于非活动期和对照者；非活动期RA患者TLR4显著高于对照组。活动期RA患者血清HMGB1与外周血CD14+单核细胞表面TLR2、TLR4相对蛋白含量表达呈显著正相关。结论 RA患者血清HMGB1及外周血单核细胞表面TLR2、TLR4的表达可能与RA病情发生发展有关，且具协同作用。

Expression and significance of HMGB1 and TLR2/TLR4 in rheumatoid arthritis

Objecive To explore the expressions of HMGB1 and TLR2/TLR4 in the patients with rheumatoid arthritis. Methods To select 29 active RA, 20 inactive RA and 18 controls.The expression of HMGB1 in serum of these cases were detected by ELISA. The expression of TLR2 and TLR4 on CD14+ monocytes in peripheral blood were detected by FCM. Results The HMGB1 content and the expression rate and relative protein quantification of TLR2 and TLR4 on CD14+ monocytes in active RA was significantly higher than inactive group and controls; The expression of and relative protein quantification of TLR4 CD14+ monocytes in inactive group is higher than controls. The level of HMGB1 in serum in active RA was positively correlated with the relative protein quantification of TLR2 and TLR4. Conclusion The expression of HMGB1 and TLR2/TLR4 in RA may be associated with the occurrence and development of RA and synchronous in rheumatoid arthritis.