肝癌组织端粒酶活性表达及DNA含量的研究

目的研究肝癌组织、癌旁组织端粒酶活性的表达及DNA含量的变化.方法取肝癌手术标本30例,每例均取癌组织、癌旁肝组织及外侧切缘肝组织.采用PCR-ELISA法检测其端粒酶的活性;流式细胞仪技术检测细胞DNA含量,DNA倍体及细胞分期.结果肝癌组织端粒酶阳性率为76.7%(23/30),癌旁组织端粒酶阳性率为16.7%(5/30),外侧切缘肝组织无阳性(0/30),肝癌组织与癌旁组织及外侧切缘肝组织端粒酶的表达有显著差异(P《0.01).癌旁组织与外侧切缘肝组织端粒酶的表达也有显著差异(P《0.05).肝癌组织与外侧切缘肝组织DNA异倍体表达率有显著差异(P《0.05).端粒酶阳性及阴性组间DNA异倍体率、DNA指数(DI)、S期细胞比率(SPF),细胞增殖指数(PI)未见明显变化.结论端粒酶与肿瘤的恶性行为存在一定的密切关系,其作为肝癌诊断的分子标志具有一定的价值.同时检测DNA倍体类型可进一步提高其临床参考价值.

Telomerase activity and DNA in liver cancer

Objective To investigate the telomerase activity and change of DNA in liver cancer.Methods telomerase activity was analyzed in 30 1iver cancers using PCR-ELISA;DNA gel electrophoresis and flow cytometry(FCM)were detected.Results Of these patients,the positive rate of telomerase activity was 76.7%(23/30),in cancerous tissues,16.7%(5/30)in juxtacancerous tissue and no positive expression of telomerase(0/30)in liver tissues.The telomerase activity between cancerous tissues and juxtacancerous tissues and liver tissues was significantly difference(P<0.01).The telomerase activity between juxtacancerous tissue and liver tissues was significantly difference(P<0.05).DNA between cancerous tissues and liver tissues was significantly difference(P<0.05)DNA,DI,SPF in tissues between positive and negative of telomerase activity have not change.Conclusion The telomerase activity with detected DNA would be a diagnosis marker for liver cancer