HPLC法同时测定元胡止痛片中3种活性成分的含量

目的:建立以高效液相色谱法同时测定元胡止痛片中延胡索乙素、欧前胡素和异欧前胡素含量的方法。方法:色谱柱为Zorbax Extend-C18(250mm×4.6mm,5μm),流动相为乙腈-0.1%磷酸溶液(三乙胺调pH6.5,梯度洗脱),流速为1mL·min-1,柱温为25℃。结果:延胡索乙素、欧前胡素、异欧前胡素的检测浓度分别在10.2～51.0、2.5～12.5、2.2～11.0μg·mL-1(均r=0.9999)范围内与各自峰面积积分值呈良好的线性关系;三者加样回收率分别为97.89%、101.77%、99.04%,RSD分别为0.8%、1.4%、1.3%(均n=9)。结论:本方法简便、快速、准确,可用于元胡止痛片的质量控制。

Determination of Three Active Components in Yuanhu Zhitong Tablet By HPLC

OBJECTIVE： To develop an HPLC method for simultaneous determination of tetrahydropalmatine, imperatorin and isoimperatorin in Yuanhu zhitong tablet. METHODS： The sample was separated on A Zorbax Extend - C18 column （250 mm ×4.6 mm, 5 μm） at column temperature of 25℃ . The mobile phase consisted of acetonitrile - 0.1% phosphoric acid solution （adjusted pH to 6.5 by triethylamine, gradient elution） at a flow rate of 1 mL·min^-1. RESULTS： The linear ranges of tetrahydropalmatine, imperatorin and isoimperatorin were 10.2--51.0μg ·mL^-1（ r - 0.999 9）, 2.5- 12.5μg·mL^-1（ r = 0.999 9） and 2.2-11.0μg·mL^-1（ r = 0.999 9） respectively, and their average recovery rates were 97.89% （RSD = 0.8%, n = 9）, 101.77% （RSD = 1.4%, n = 9） and 99.04% （RSD - 1.3%, n = 9）, respectively. CONCLUSION： The method is simple, rapid, accurate, and applicable for the quality control of Yuanhu zhitong tablet.