大肠杆菌菌种空间变异的研究

为研究微生物菌种在空间条件下产生的变异，在利用我国发射的返回式卫星上，搭载了适用于突变研究的大肠杆菌菌株ＣＳＨ１０８、Ａ３和Ａ２，使用了三种搭载方式。

Mutation Induced by Space Conditions in Escherichia coli Strains.

To study the mutagenesis of microorganism under space conditions, three Escherichia coli strains CSH108, A2 and A3, were flown on board the Chinese recoverable satellite (JB1 - B9611020) in Oct . 1996. Three ways of sample boarding were designed . After recovery, survival and frequency of lacI- mutation and Arg+ reversion were determined in the flight strains. The results showed that: E. coli strains could survive after the flight conditions. The lacI- mutation frequency of A3 strain in the biocabin was sixty - seven times that of the ground control, the Arg+ reversion frequency of CSH108 in the lead chamber, was ten times that of the ground control; and among the revertants the mutation frequency of non-sense suppressor increased remarkably. The results indicate that the space conditions may greatly enhance the mutation frequency of certain genes in the microorganism and may provide an effective method for microorganism breeding.