High Yield of Wax Ester Synthesized from Cetyl Alcohol and Octanoic Acid by Lipozyme RMIM and Novozym 435 |
| |
| Authors: | Chia-Hung Kuo Hsin-Hung Chen Jiann-Hwa Chen Yung-Chuan Liu Chwen-Jen Shieh |
| |
| Affiliation: | 1.Biotechnology Center, National Chung Hsing University, 250 Kuo-kuang Road, Taichung 402, Taiwan; E-Mail: ;2.Department and Graduate Program of Bioindustry Technology, Dayeh University, 168 University Road, Chang-Hwa, 515, Taiwan; E-Mail: ;3.Graduate Institute of Molecular Biology, National Chung Hsing University, 250 Kuo-kuang Road, Taichung, 402, Taiwan; E-Mail: ;4.Department of Chemical Engineering, National Chung Hsing University, 250 Kuo-kuang Road, Taichung, 402, Taiwan; E-Mail: |
| |
| Abstract: | Wax esters are long-chain esters that have been widely applied in premium lubricants, parting agents, antifoaming agents and cosmetics. In this study, the biocatalytic preparation of a specific wax ester, cetyl octanoate, is performed in n-hexane using two commercial immobilized lipases, i.e., Lipozyme® RMIM (Rhizomucor miehei) and Novozym® 435 (Candida antarctica). Response surface methodology (RSM) and 5-level-4-factor central composite rotatable design (CCRD) are employed to evaluate the effects of reaction time (1–5 h), reaction temperature (45–65 °C), substrate molar ratio (1–3:1), and enzyme amount (10%–50%) on the yield of cetyl octanoate. Using RSM to optimize the reaction, the maximum yields reached 94% and 98% using Lipozyme® RMIM and Novozym® 435, respectively. The optimum conditions for synthesis of cetyl octanoate by both lipases are established and compared. Novozym® 435 proves to be a more efficient biocatalyst than Lipozyme® RMIM. |
| |
| Keywords: | optimization wax esters lipase esterification Rhizomucor miehei Candida antarctica |
|
|
