不同亚型乙型病毒性肝炎患者T细胞亚群调查研究

目的研究不同亚型乙型肝炎患者外周血T淋巴细胞亚群变化及其临床意义。方法选择40例慢性活动性乙型肝炎、30例乙型肝炎病毒携带者、30例乙型肝炎肝硬化、20例急性乙型肝炎患者和30例健康对照者,采用流式细胞仪检测各组外周血T淋巴细胞亚群的表型和频率。结果与正常对照组比较,慢性活动性肝炎、肝炎肝硬变患者外周血CD4 、CD4 /CD8 比值均有显著性下降(P<0.01),以肝炎肝硬变下降最显著,慢性病毒携带者肝炎、急性乙型肝炎外周血T细胞亚群数量和比值变化不明显(P>0.05)。HBV DNA病毒载量与患者外周血CD4 T细胞数量无相关性(P>0.05)。结论慢性活动性乙型肝炎和肝硬化患者的细胞免疫功能降低,其下降程度与病毒复制水平无相关性。

Investigation of the T Lymphocyte Subset Population from different Subtype Patients with Hepatitis Virus B Infection

Objective To study on T lymphocyte subset population of peripheral blood from patients with different subtype HBV infection.Methods Selection of 30 cases with asymptomatic carriers(AsC),40 cases with chronic active hepatitis virus B(CAH),30 cases with liver cirrhosis(LC),20 cases with acute hepatitis virus B(AHB),30 normal subjects(NS),T lymphocyte subset population of peripheral blood of the five groups were detected by flow cytometry.Results The proportion of CD3 CD4 T cell and the ratio of CD4/CD8 of peripheral blood from patients with CAH and LC were significantly decreased compared with those from NS(P<0.01).But those from AsC and AHB had no significantly change in statistics(P>0.05).These was no correlation between HBV DNA viral load and T lymphocyte subset population counts.Conclusions The cellular mediated immune functions of patients with CAH and LC were lowered compared with healthy controls.The decrease in CD4 T cell count had no correlation between HBV DNA viral load.