紫背天葵药材质量标准研究

目的建立紫背天葵药材的质量控制标准。方法用聚酰胺薄层色谱法鉴别矢车菊素-3-O-葡萄糖苷;按照《中国药典》方法测定多批药材的水分、总灰分、酸不溶性灰分和水溶性浸出物,确定以上检查项目的限度;用高效液相色谱法进行药材中矢车菊素-3-O-葡萄糖苷的含量测定。结果聚酰胺薄层色谱斑点清晰、专属性强、重现性好;药材水分、总灰分、酸不溶性灰分分别不得过14%、15%、2.5%,沸水煮浸法水溶性浸出物量不得少于13%;紫背天葵药材含矢车菊素-3-O-葡萄糖苷不得少于0.5%。结论建立的质量标准适用于评价紫背天葵药材的质量。

Study on the quality standard of Begonia fimbristipulata Hance

Objective To establish the quality control standard of Begonia fimbristipulata Hance.Methods Cyanidin-3-O-glucoside was identified by polyamide TLC.The water content,total ash,acid insoluble ash and water-soluble extract of multiple batches were determined according to the Chinese Pharmacopoeia,and their limits were set.And HPLC was performed to determine the content of cyanidin-3-O-glucoside.Results Clear spots were observed in the polyamide TLC with high specificity and good repeatability.The water content,total ash,acid insoluble ash and water-soluble extract for B.fimbristipulata should be less than 14%,15%,2.5% and 13% individually.The content of cyanidin-3-O-glucoside in B.fimbristipulata should not be less than 0.5%.Conclusion The quality control standard established in this study was suitable for evaluate the quality of B.fimbristipulata.