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离子交换色谱法测定枇杷果实和叶片中的有机酸
引用本文:陈发兴,刘星辉,林华影,陈立松. 离子交换色谱法测定枇杷果实和叶片中的有机酸[J]. 福建农林大学学报(自然科学版), 2004, 33(2): 195-199
作者姓名:陈发兴  刘星辉  林华影  陈立松
作者单位:1. 福建农林大学亚热带果树研究所,福建,福州,350002 ;福建农林大学园艺学院,福建,福州,350002
2. 福建农林大学园艺学院,福建,福州,350002
3. 福州市疾病预防控制中心,福建,福州,350004
基金项目:福建省自然科学基金资助项目(B0310008).
摘    要:用离子交换色谱法对枇杷有机酸进行有效分离与测定,EG40淋洗液在线发生器能降低背景电导,获得较好的重现性和平稳的基线.测定结果表明,枇杷果实和叶片中有机酸组分和含量有较大差别.果实中以苹果酸为主,其次为奎尼酸和酒石酸,而柠檬酸、草酸和顺乌头酸等含量较低.叶片中以奎尼酸为主,苹果酸、柠檬酸和草酸含量很低.

关 键 词:枇杷  果实  叶片  有机酸  离子交换色谱
文章编号:1006-7817(2004)02-0195-05
修稿时间:2004-02-09

Determination of the organic acids from the fruit and leaf of loquat by ion-exchange chromatography
CHEN Fa-xing. Determination of the organic acids from the fruit and leaf of loquat by ion-exchange chromatography[J]. Journal of Fujian Agricultural and Forestry University, 2004, 33(2): 195-199
Authors:CHEN Fa-xing
Affiliation:CHEN Fa-xing~
Abstract:Organic acids in loquat samples were adequately determined by ion-exchange chromatography. The performing gradient elution with EG40 eluent generator was used to obtain a flat baseline and better reproducibility. Results showed that the malic acid was the top organic acid in fruit; the seconds were quinic acid and tartartic acid. The others as citric, oxalic, cis-aconitic acid were little in fruit. In leaves, the main organic acid was quinic acid, while citric, malic and oxalic acid were little.
Keywords:loquat  fruit  organic acid  high-performance ion-exchange chromatography (HPIC)
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