逍遥散对雌性兔膝关节软骨组织及性激素水平的影响

目的：探讨逍遥散对雌性兔性激素水平及膝关节软骨组织损伤的影响。方法：40只新西兰兔通过手术方法进行膝关节失稳型造模,1周后随机分为中药组、西药组、混合组和对照组各10只,驱赶行走4周,分别灌药或灌生理盐水8周。造模后12周末处理动物,血清检测雌二醇、睾丸酮含量;HE染色、甲苯胺蓝染色,光镜观察膝关节软骨组织并进行Mankin分级评分。结果：4组动物性激素含量无统计学意义（P〉0.05）;对照组软骨组织中度损伤,中药组、西药组和混合组软骨组织轻度损伤,结果比较有统计学意义。结论：逍遥散可明显减轻兔膝关节软骨组织损伤,对雌性兔性激素水平的影响有待进一步研究。

Study on Influence of Xiaoyao Powder on Female Rabbit Sex Hormone Levels and Knee Cartilage Tissue

Objective ： To explore the influence of Xiaoyao Powder on sex hormone levels and knee cartilage tissue damage in rabbits. Method： 40 New Zealand rabbits were modeled through operation forknee joint instability.A week after modeled, they were randomly divided into four groups ： traditional Chinese medicine group, Western medicine group, mixed group and control group, 10 for each group.Each rabbit had been driven to walk for 4 weeks, then medicine or saline perfusion for 8 weeks.12 weeks after, the animals were killed and the contents of serum estradiol and testosterone were detected ; the knee joint Cartilage was observed by light microscope after HE and toluidine blue stained, and scored by Mankin scale. Result ： The sex hormones of four groups of animals showed no statistical significance （ P〉0.05 ）; cartilage degeneration in a serious for control group, mild degeneration for traditional Chinese medicine group, Western medicine group and mixed group, there was significant difference for the result. Conclusion ： Xiaoyao Powder could significantly relieve the damage of cartilage tissue of rabbit knee, and the effect of sex hormone levels in rabbits remains to be further confirmed.