高压二氧化碳对人冠状病毒（hCoV-229E）的消杀效果

目的：探究高压二氧化碳（HPCD）对人冠状病毒229E(hCoV-229E)的消杀效果。方法：采用HPCD在不同温度（10、25、37℃）、压力（6.3、10 MPa）和时间（15、30 min）条件下，对hCoV-229E进行消杀处理。结果：与不加压相同温度处理的对照组相比，HPCD在10℃、6.3 MPa条件下对50 mL离心管中的病毒液处理30 min后，病毒滴度显著降低（P<0.05）；在37℃、10 MPa条件下对50 mL离心管中的病毒液处理15 min后，病毒滴度极显著降低（P<0.01）；在37℃、10 MPa条件下对表面含有病毒的三文鱼肉、虾壳、聚乙烯类包装材料处理15 min，病毒滴度显著降低（P<0.05）。结论：HPCD在37℃、10 MPa条件下处理15 min，可以有效消杀食品（三文鱼肉和虾壳）及聚乙烯类包装材料表面的hCoV-229E。

Disinfection Effect of High-pressure Carbon Dioxide on Human Coronavirus (hCoV-229E)

Objective: Exploring the disinfection effect of high-pressure carbon dioxide (HPCD) on human coronavirus 229E (hCoV-229E). Methods: The human coronavirus 229E (hCoV-229E) was treated by HPCD at different temperatures (10, 25, and 37 ℃) and pressures (6.3 and 10 MPa) for different time (15 and 30 min). Result: Compared with the control groups under the corresponding temperatures, the virus titer in 50 mL tube treated with HPCD at 10 ℃ and 6.3 MPa for 30 min was significantly decreased (P<0.05). The virus titer was also significantly reduced after treatment with HPCD at 37 ℃ and 10 MPa for 15 min (P<0.01). Moreover, the virus titers inoculated on the surfaces of salmon meat, shrimp shell, and polyethylene packing materials were all significantly decreased after HPCD treatment at 37 ℃ and 10 MPa for 15 min as compared with the corresponding control groups (P<0.05). Conclusion: HPCD treatment at 37 ℃ and 10 MPa for 15 minutes could effectively disinfect hCoV-229E on the surface of food (salmon meat and shrimp shell) and polyethylene packaging materials.