人工培养基上稻曲病菌拟菌核诱导的初步研究

稻曲病菌在水稻孕穗期侵染水稻小花,在水稻灌浆后期只在个别稻曲球上形成菌核。因此,稻曲病菌菌核分化与发育基因功能研究的相关试验难度较高。为了探究稻曲病菌在人工培养基上能否形成菌核,本文利用低温或杀菌剂环境对稻曲病菌进行了筛选。结果发现,在81个菌株中有13个在人工培养基上形成拟菌核组织,且它们可在低温处理时稳定地产生拟菌核组织。这些拟菌核组织在结构上与菌核类似,但体积较小且组织较为疏松,无法像菌核一样越冬和完成有性生殖。

Primary study on induction of the para-sclerotia of Villosiclava virens in artificial medium

Villosiclava virens, the pathogen of rice false smut, attacks rice florets at rice booting stage, and forms sclerotia in few false smut balls at the late stage of rice filling. This makes it difficult to assay the bio-function of the genes related to sclerotial differentiation and development. In this study, the attempt to induce the sclerotia formation in artificial medium was carried out with stress by tricyclazole, a fungicide, and low temperature. Among 81 isolates of the pathogen in the test, the para-sclerotia appeared in 13 isolates. The para-sclerotia were similar to the sclerotia in false smut balls both in morphology and ultra-structure, although they were small in size and loose in structure. However, they could not overwinter and complete the sexual reproduction.