苹果褪绿叶斑病毒辽宁分离物生物学和基因组研究

正苹果褪绿叶斑病毒(Apple chlorotic leaf spot virus,ACLSV)属于乙型线形病毒科(Betaflexiviridae)纤毛病毒属(Trichovirus),基因组为正义单链RNA,含有约7 500个核苷酸,编码3个部分重叠的开放阅读框。目前从Gen Bank数据库可以检索到19个ACLSV分离物的基因组序列。该病毒侵染仁果与核果,如苹果和桃,在苹果栽培种上不引起症状,在特定指示植物上引起症状,如俄罗斯苹

Biology and complete genome of Apple chlorotic leaf spot virus Liaoning isolate

In the present work, we studied the genomic and biological properties of Apple chlorotic leaf spot virus (ACLSV) isolate XC-HF. The leaves of Malus domestica cv. Hanfu collected from Xingcheng, Lianoning province were used as plant material. The samples were subjected to RT-PCR and RACE techniques to amplify the complete genome of ACLSV XC-HF, followed by sequencing. The genome of ACLSV XC-HF consists of 7 557 nucleotides, encoding three overlapping open reading frames (ORFs) and shared nucleotide identities of 68.9%-84.4% with the other 19 ACLSV isolates published in GenBank. Phylogenetic analysis based on the complete genome sequences indicated that ACLSV XC-HF was located in the group JB. Recombination analysis suggested a recombinant event at 1-338 nts of ACLSV XC-HF. The ACLSV XC-HF was inoculated to Chenopodium amaranticolor by sap inoculation and to Malus sylvestris cv. R12740-7A by double budding, respectively, and induced symptoms of chlorosis and slight leaf roll on infected plants.