| PAR-吖啶黄能量转移荧光猝灭法测定痕量铁(Ⅱ)的研究 |
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| 引用本文： | 鲍所言,王桂华,刘保生,孙汉文,黄永章,石生勋.PAR-吖啶黄能量转移荧光猝灭法测定痕量铁(Ⅱ)的研究[J].光谱学与光谱分析,2001,21(1):87-89. |
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| 作者姓名： | 鲍所言 王桂华 刘保生 孙汉文 黄永章 石生勋 |
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| 作者单位： | 1. 河北大学化学系,
2. 河北大学理化分析中心, |
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| 摘 要： | 对 4 (2 吡啶偶氮 ) 间苯二酚 (PAR)与吖啶黄间荧光能量转移进行了探讨 ,研究了其能量转移的最佳条件 ,并应用该体系测定痕量铁 (Ⅱ ) ,由此建立了能量转移荧光测定痕量铁的新方法。在λex/em =46 5nm/5 0 5nm ,十二烷基硫酸钠 (SDS)作用下 ,pH =9 2时 ,吖啶黄与PAR 铁 (Ⅱ )络合物间能发生有效能量转移 ,使吖啶黄的荧光猝灭。铁的量在 0～ 10 μg·L-1范围内呈良好的线性关系。最低检出限 0 0 6 μg·L-1。该方法用于水样、发样中痕量铁的测定 ,结果满意。
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| 关 键 词： | 吖啶黄 4－（2－吡啶偶氮）－间苯二酚 PAR 能量转移 荧光猝灭 铁 痕量分析 水 头发 荧光分析 |
| 修稿时间： | 1999年11月26 |
Study on the Energy Transfer Fluorescence Quenching among Combination of Acridine Yellow-PAR Determination of Trace Iron |
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| Suoyan BAO,Guihua WANG,Baosheng LIU,Hanwen SUN,Yongzhang HUANG,Shengxun SHI.Study on the Energy Transfer Fluorescence Quenching among Combination of Acridine Yellow-PAR Determination of Trace Iron[J].Spectroscopy and Spectral Analysis,2001,21(1):87-89. |
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| Authors: | Suoyan BAO Guihua WANG Baosheng LIU Hanwen SUN Yongzhang HUANG Shengxun SHI |
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| Affiliation: | Department of Chemistry, Hebei University, 071002 Baoding. |
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| Abstract: | An energy transfers technique between 4-(2-Pyridylazo) resorcinol(PAR) and acridine yellow is studied, and optimum experimental conditions of energy transfer is done as well. It is found that in the aqueous solution of sodium lauryl sulfate, NH3.H2O-NH4Cl buffer solution at pH = 9.2, the energy transfers from acridine yellow to iron(II)-PAR complexes. So a new method of fluorometric energy transfers determination of trace iron(II) by acridine yellow--PAR was described. The range of determination of iron is 0-10 micrograms.L-1. The detection limit for iron is 0.06 microgram.L-1. The method has been applied to the determination of iron in water sample and hair with satisfactory results. |
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| Keywords: | Acridine yellow PAR Energy transfer Fluorescence quenching Iron |
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