门静脉高压大鼠胃黏膜及微血管增生程度的初步研究

目的研究门静脉高压性胃病胃黏膜及微血管增生情况.方法采用改良四氯化碳吸入法建立大鼠肝硬化门静脉高压的胃黏膜病变模型,通过光学显微镜研究其胃黏膜及微血管病理变化并利用免疫组织化学染色研究增殖细胞核抗原(PCNA)和胃黏膜微血管密度(MVD)的分布.结果光镜下门静脉高压时大鼠胃黏膜呈现全胃黏膜和微动静脉充血,毛细血管扩张、膨胀;门静脉高压症大鼠胃黏膜PCNA表达较正常明显增高(P＜0.01),PCNA表达集中在黏膜固有层;门静脉高压症大鼠胃黏膜微血管数目较正常明显增多(P＜0.01).结论肝硬化门静脉高压症大鼠胃黏膜细胞增殖明显(可以从PCNA的表达及分布规律中体现出来),胃黏膜微血管数目亦显著增多.

A preliminary study on proliferation of gastric mucosal cells and microvessels in portal hypertensive rats induced by CCl4

Objective To study the proliferative extents of cells and microvessels of gastric mucosa in portal hypertensive rats. Methods Models of portal hypertension were induced by carbon tetrachloride inhalation. The pathological changes of microvessels of gastric mucosa were detected by microscopy. At the same time, expressions of mucosoal PCNA and micro vascular density (MVD) were determined by immunohistochemistry assay. Results 1. Microvessels of gastric mucosa showed dilatation of venules, arterioles and capillaries along with extensive edema in lemina propia and submucosa. 2. Overexpressions of PCNA were detected in mucosa gastric mucosa, and the increased PCNA was mostly presented in the lemina propia. 3. The MVD was significantly higher than that of the normal mucosa(P<0.01). Conclusion Mucosal cells are proliferated magnificantly in portal hypertensive rats induced by CCl_4, which is demonstrated by the increase of the distribution of PCNA and the MVD.