游离脂肪酸对大鼠肾小球系膜细胞增殖和细胞周期的影响

目的：探讨游离脂肪酸（FFAs）对体外培养的大鼠肾小球系膜细胞增殖和生长周期的影响。方法：用不同浓度的游离脂肪酸处理大鼠HBZY-1细胞株（即大鼠肾小球系膜细胞）24h～72h。采用噻唑蓝比色（MTT）法检测内皮细胞增殖情况,流式细胞术（FCM）分析法测定细胞周期变化。结果：游离脂肪酸可抑制HBZY-1细胞的生长增殖（与对照组比较,P〈0.01）,且这种抑制作用具有剂量和时间依赖性;游离脂肪酸作用于HBZY-1细胞24h、48h、72h,细胞周期发生明显改变,G1期细胞数增多,S期细胞数减少（与对照组比较,P〈0.01）。结论：游离脂肪酸可通过停滞细胞生长于G1期,抑制大鼠肾小球系膜细胞的生长增殖。

Effect of Free Fatty Acids on Cell Proliferation and Cell Cycle in Rat Mesangial Cells

Objective:To investigate the effects of free fatty acids(FFAs)on proliferation and cell cycle of rat Mesangial cells(MC)in vitro.Methods:Cultured rat Mesangial cells line(HBZY-1)were incubated with various concentrations of FFAs for 24h-72h.Inhibition of proliferation was measured by colorimetric 3-[4,5-dimethyl thiazol-2-yl]-2,5-diphenyltetrazolium bromide(MTT)assay.The change of the phases of cell cycle was analyzed by flow cytometry.Results:FFAs inhibited the proliferation of HBZY-1 cells in a dosage-and time-dependent manner by MTT assay(P<0.01 vs control respectively).The fraction of G1 phase was increased,and that of S phase decreased in HBZY-1 cells treated with different concentrations of FFAs for 24 h,48 h,72 h(P<0.01 vs control respectively).Conclusion:FFAs could inhibit proliferation of rat Mesangial cells in vitro by arresting cells growth in G1 phase.