| 哈蟆油发酵乳中蛋白质及氨基酸含量分析 |
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| 引用本文： | 刘同帅,邱智东,翁丽丽,郝洁,翁砚. 哈蟆油发酵乳中蛋白质及氨基酸含量分析[J]. 长春中医药大学学报, 2016, 32(2). DOI: 10.13463/j.cnki.cczyy.2016.02.014 |
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| 作者姓名： | 刘同帅 邱智东 翁丽丽 郝洁 翁砚 |
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| 作者单位： | 长春中医药大学,长春,130117;白求恩医科大学制药厂,长春,130012 |
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| 基金项目： | 吉林省科技发展计划项目(20120912)。 |
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| 摘 要： | 目的：建立哈蟆油发酵乳中蛋白质及氨基酸含量分析方法。方法运用考马斯亮蓝法测定哈蟆油发酵乳中蛋白质含量，运用氨基酸自动分析仪测定哈蟆油发酵乳中氨基酸的含量。结果3批哈蟆油发酵乳蛋白含量分别为8.0501、8.0930、7.7582 mg/mL。平均总氨基酸的含量为15.3205 mg/mL。结论哈蟆油发酵乳中含有合成人体所需蛋白质及丰富的氨基酸。
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| 关 键 词： | 蛤蟆油发酵乳 考马斯亮蓝法 蛋白质 氨基酸 |
Analysis of protein and amino acid content of Oviductus Ranae in fermented milk |
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| LIU Tongshuai,QIU Zhidong,WENG Lili,HAO Jie,WENG Tan. Analysis of protein and amino acid content of Oviductus Ranae in fermented milk[J]. Journal of Changchun College of Traditional Chinese Medicine, 2016, 32(2). DOI: 10.13463/j.cnki.cczyy.2016.02.014 |
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| Authors: | LIU Tongshuai QIU Zhidong WENG Lili HAO Jie WENG Tan |
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| Abstract: | Objective To establish a method for analysis of protein and amino acidcontent of Ranae Oviductus fermented milk. Methods Using Coomassie bright blue for determination of Ranae Oviductus fermented milk protein content,determination of the content of amino acids by automatic amino acid analyzer. Results The three batch of Ranae Oviductus fermented milk protein content were 8. 050 1,8. 093 0,7. 758 2 mg/mL. The average total amino acid content was 15. 320 5 mg/mL. Conclusion Ranae Oviductus in fermented milk containing synthetic human body needs protein rich amino acids. |
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| Keywords: | Ranae Oviductus fermented milk Kaumas Bradford method protein amino acid |
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