替硝唑棒对牙周炎和冠周炎病原菌的抗菌作用

目的:评价替硝唑棒对牙周炎和冠周炎病原菌的抗菌作用。方法:(1)采用厌氧菌药物敏感试验的二倍琼脂稀释法测定替硝唑和甲硝唑对200株临床分离株的MIC50和MIC90。(2)采用厌氧菌培养方法测定51例牙周炎和冠周炎患者局部应用替硝唑和甲硝唑前后,牙周炎病原菌和冠周炎病原菌的变化。结果:替硝唑棒剂对牙龈卟啉单胞菌、中间普氏菌、梭杆菌和消化链球菌具有明显抗菌作用,其MIC50和MIC90分别为0.25、0.25、0.25、8 mg/L和0.5、0.5、1、8mg/L;其清除率为94.4％～100％,细菌数量均有明显减少。替硝唑对牙周炎和冠周炎病原菌的抗菌活性明显强于甲硝唑(P<0.05)。结论:局部应用替硝唑棒可明显抑制牙周炎和冠周炎的病原菌。

A Study of Antimicrobial Effects of Tinidazole against Pathogens of Periodontitis and Pericoronitis

Objective: The purpose of this study was to evaluate the antimicrobial efficacy of tinidazole. Methods: A total of 51 patients with periodontitis and pericoronitis were selected for this study. Among these patients, 25 of them were treated by local application of metronidazole stilus, and 26 of them were treated with tinidazole stilus. During the same period, patients were sampled, cultured and the bacteria were grown in an anaerobic chamber for 5 ~ 7 days. The MIC50 and MIC90 of tinidazole and metronidazole were determined by using two-fold agar dilution technique against 200 wide-type isolates isolated from patients with periodontitis and pericoronitis.Results: Tindazole had stronger antibacterial activities against Porphyromonas gingivalis, Prevotella, intermedia., Fusobacteruan spp. and Peptostreptococcus spp. The MIC50 and MIC90 were 0.25, 0.25, 0.25, 8and0.5, 0.5, 1, 8 mg/L respectively. The elimination rate was 94.4- 100% . The antimicrobial activity of Tinidazole was significantly stronger than metronidazole ( P < 0.05) . Conclusion: Tinidazole stilus can be applied locally in the treatment of periodontitis and pericoronitis.