| 使用切片原位杂交定位定量BaP与EE2对卵形芳香化酶表达影响的探讨 |
| |
| 引用本文： | 刘建宇,杨景峰,高原,解彦炯,王思珍,董武.使用切片原位杂交定位定量BaP与EE2对卵形芳香化酶表达影响的探讨[J].内蒙古民族大学学报(自然科学版),2009,24(1):48-50. |
| |
| 作者姓名： | 刘建宇 杨景峰 高原 解彦炯 王思珍 董武 |
| |
| 作者单位： | 内蒙古民族大学动物科技学院,内蒙古,通辽,028042 |
| |
| 基金项目： | 教育部新世纪优秀人才支持计划，教育部留学回国人员科研启动基金 |
| |
| 摘 要： | 通过对青鲻的染毒观测，使用组织切片技术与原位杂交相结合的方法，定位定量苯并（a）芘（BaP）与17α-乙炔基雌二醇（EE2）对初级卵母细胞中卵形芳香化酶表达影响的研究，来确定环境污染物质对生殖系统相关基因表达的影响．结果表明：BaP降低了卵形芳香化酶的表达强度（P〈0．05），而EE2增强了卵形芳香化酶的表达强度（P〈0．05）．
|
| 关 键 词： | 苯并(a)芘 17α-乙炔基雌二醇 卵形芳香化酶 |
The Investigate of Expression Influencing of Localization and Quantitation BaP and EE2 to Ovoid Aromatizing Enzyme by Use of Sections and Hybridization in Situ |
| |
| LIU Jian-yu,YANG Jing-feng,GAO Yuan,XIE Yan-jiong,WANG Si-zhen,DONG Wu.The Investigate of Expression Influencing of Localization and Quantitation BaP and EE2 to Ovoid Aromatizing Enzyme by Use of Sections and Hybridization in Situ[J].Journal of Inner Mongolia University for the Nationalities(Natural Sciences),2009,24(1):48-50. |
| |
| Authors: | LIU Jian-yu YANG Jing-feng GAO Yuan XIE Yan-jiong WANG Si-zhen DONG Wu |
| |
| Affiliation: | (College of Animal and Technology, Inner Mongolia University for Nationalities, Tongliao 028042, China ) |
| |
| Abstract: | Through Fundulus heteroclitus exposed to the poison, the investigate of expression influencing of localization and quantitation benzo（a）pyrene （BaP） and 17 α-ethinylestradiol （EE2） to ovoid aromatizing enzyme in primary oocyte by use of sections and hybridization in situ, to determine that the influencing of related gene expression in genital system by environmental pollution. The result indicated that BaP reduced the expression intensity of ovoid aromatizing enzyme （P〈0. 05）, but EE2 strengthened the expression intensity of ovoid aromatizing enzyme （P〈0.05）. |
| |
| Keywords: | Bzo（a）pyrene 17α-ethinylestradiol Ovoid aromatizing enzyme |
| 本文献已被 维普 万方数据 等数据库收录！ |
