Demonstration of a macromolecule cross-reacting with antibodies to luteinizing hormone releasing hormone and its tissue distribution |
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| Authors: | Ayalla Barnea John C. Porter |
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| Affiliation: | Cecil H. and Ida Green Center for Reproductive Biology Sciences The Departments of Obstetrics and Gynecology and Physiology The University of Texas Health Science Center at Dallas Southwestern Medical School, Dallas, Texas 75235 USA |
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| Abstract: | Hypothalamic cytosol contains a macromolecule which cross-reacts with antibodies to luteinizing hormone releasing hormone (LRH). This cross-reacting material (macro-CRM) is insoluble in methanol or acid ethanol, and its molecular weight is about 70,000. Macro-CRM is also found in cytosols of extra-hypothalamic regions of the brain, liver, kidney, spleen, and skeletal muscle. Plasma contains only marginal amounts of macro-CRM. This substance inhibits the binding of [125I]LRH to LRH antibodies in a reversible, competitive manner. |
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| Keywords: | EtOH ethanol LRH luteinizing hormone releasing hormone macro-CRM macromolecular cross-reacting material MeOH methanol PBS .01M phosphate buffer and .14M NaCl, pH 7.0 TRH thyrotropin releasing hormone |
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