抗独特型抗体作为日本血吸虫病诊断抗原的研究

本研究用单克隆抗独特型抗体ＮＰ３０作为抗原，进行ＥＬＩＳＡ检测日本血吸虫血清抗体，与用肠相关抗原ＧＡＡ和可溶性虫卵抗原ＳＥＡ对照，检测１００份急性病人血清，ＮＰ３０检出率为９５％；ＧＡＡ为９２％；ＳＥＡ为９７％，三埂无显著差别，５２例慢性病人中ＮＰ３０与ＧＡＡ检出率均为７３．１％，但与ＳＥＡ９４．２％有显著差别，在４０例已治愈病人中，ＮＰ３０转阴率为４２．５％与ＧＡＡ的４５％无差别。但ＳＥＡ仅为１

Study on the Monoclonal Anti-idiotypic Antibody as an Antigen Used In The Diagnosis of Schistosomiasis Japonica

In this study the monoclonal anti-idiotypic antibody NP30 was used as "antigen reagent" for the antibody detection by ELISA with associated antigen (GAA) and soluble egg antigen (SEA) as control. In the 100 individuals sera from acute infection. NP30 gave 95% of seropstivity,there's no remarkable diference among the three detection rate,GAA 92% and SEA 97%. In the 52 sera from chronic infection. The seropositivity with both NP30 both NP30 and GAA was 73. 1% markedly lower than with SEA 94. 2% (P<0. 01). However the conversing to negative rate which NP30 gave in the 40 sera from cured patients was 42. 5% and GAA 45%, SEA only 15%, as negative controls 50 sera were examined and 6% false seropositivity was found with NP30 and 8% with GAA, 2% with SEA, with no remakable difference of statistical. In addition, of 20 sera from individuals infected with Paragonimus westermani, only on showed positive reaction with SEA and in 10 samples from those infected with Clonorchis sinensis, also one showed positive reading with each of the three regents. Taken together it suggested that antiidiotypic antibody NP30 could be used as antigen for the diagnosis derived from schistome japonicum.